《植物生理学报》 2009, 45(7): 684-686
通信作者：刘卫群；E-mail: email@example.com；Tel: 0371-63555153
摘 要：水培65 d的烟株进行打顶后, 测定打顶和不打顶烟株上部叶中烟碱含量。分别提取根中总RNA, 以腐胺N-甲基转移酶基因(PMT)特异引物、肌动蛋白基因(actin)为内参作半定量RT-PCR分析; 同时将提取的总RNA转膜, 用地高辛标记PMTRT-PCR产物作探针, 进行Northern杂交分析的结果表明, 打顶后的上部烟叶中烟碱含量和PMT基因表达量显著增加。
关键词：烟草打顶; 腐胺N- 甲基转移酶基因(PMT); 半定量RT-PCR; Northern 杂交
Corresponding author: LIU Wei-Qun; E-mail: firstname.lastname@example.org; Tel: 0371-63555153
Abstract:The tobacco (Nicotiana tabacum) plants were topped after water cultured for 65 d and the top leaf nicotine contents of the topped and untopped were examined. Total RNA were extracted respectively from the roots of topped and untopped tobacco plants. Expression analysis of putrescine N-methyltransferase gene (PMT) was carried out by semi-quantitative RT-PCR with the tobacco actin as control and by Northern blotting with the digoxin-labelled PMT RT-PCR product as probe. Results showed that PMT expression and the nicotine content in the top leaf increased after topping.
Key words: tobacco topping; putrescine N-methyltransferase gene (PMT); semi-quantitative RT-PCR; Northern blotting
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