苦参和苦豆子的过氧化物酶同工酶谱分析和比较

张新中, 纪瑛*
甘肃农业职业技术学院, 兰州730020

通信作者:纪瑛;E-mail: lzjiying2005@163.com;Tel: 0931-4908708

摘 要:

采用聚丙烯酰胺凝胶电泳技术分析和比较了5个不同产地的苦参和1种苦豆子的过氧化物酶同工酶活性。结果表明: 不同产地苦参品种和苦豆子的过氧化物酶同工酶谱迁移率在0.14~0.28之间, 共6条酶谱带。聚类分析表明, 苦参和苦豆子 的亲缘关系较远, 而不同产地的苦参种质也存在一定的差异, 可分为3类: 甘肃成县栽培的苦参与甘肃岷县栽培的苦参为一 类, 河南卢氏野生苦参单独为一类, 河北承德野生苦参和甘肃成县野生苦参为一类。

关键词:苦参; 苦豆子; 过氧化物酶同工酶; 聚类分析

收稿:2009-03-18   修定:2009-06-16

资助:甘肃省科技攻关项目(2GS064-A43-019-08)。

Analyses and Comparison on Peroxidase Isoenzymes of Sophora flavescens Ait. and Sophora alopecuroides L.

ZHANG Xin-Zhong, JI Ying*
Gansu Agriculture Technical and Vocational College, Lanzhou 730020, China

Corresponding author: JI Ying; E-mail: lzjiying2005@163.com; Tel: 0931-4908708

Abstract:

Experiments were conducted to analyze the peroxidase isoenzymes of Sophora flavescens and Sophora alopecuroides. The peroxidase isoenzymes of five sources of S. flavescens and S. alopecuroides were determined by polyacrylamide gel electrophoresis technique. The results indicated that the electrophoretic mobilities of the isoenzyme spectra were among 0.14–0.28. There were six peroxidase-active bands observed. According to cluster analysis on the isoenzyme spectra, S. flavescens showed a distant relationship to S. alopecuroides, and the germplasms of the five sources of S. flavescens were classified into three groups.

Key words: Sophora flavescens; Sophora alopecuroides; peroxidase isoenzyme; cluster analysis

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