水杨酸途径中的防御基因在转草酸氧化酶基因油菜中的表达

冀瑞琴1,2, 董祥柏2, 冯辉1, 高荣村2, 刘越英2, 刘胜毅2,*
1 沈阳农业大学园艺学院, 沈阳110161; 2 中国农业科学院油料作物研究所, 武汉430062

通信作者:刘胜毅;E-mail: liusy@oilcrops.cn;Tel: 027-86812896

摘 要:

采用 RT-PCR 方法检测对水杨酸依赖的信号途径中 PR-1、GLU 和 CHI 3 个防御基因在转 OXO 基因与非转基因的油 菜中表达差异的结果表明, 在转基因油菜株系中这3个基因都有不同程度的上调表达, 暗示转OXO基因的油菜抗菌核病的能力增强可能与对水杨酸依赖的信号途径受诱导有关。

关键词:对水杨酸依赖的信号途径; 防御基因; 油菜; 菌核病

收稿:2009-05-05   修定:2009-06-17

资助:国家自然科学基金(30800748)和中国博士后科学基金(20070411080)。

Expressions of Defense Genes of Salicylic Acid-Dependent Signal Pathway in Oxalate Oxidase Transgenic Oilseed Rape (Brassica napus L.)

JI Rui-Qin1,2, DONG Xiang-Bai2, FENG Hui1, GAO Rong-Cun2, LIU Yue-Ying2, LIU Sheng-Yi2,*
1College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China; 2Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China

Corresponding author: LIU Sheng-Yi; E-mail: liusy@oilcrops.cn; Tel: 027-86812896

Abstract:

Expressions of three defense genes (PR-1, GLU and CHI) of salicylic acid (SA)-dependent signaling pathway were measured by RT-PCR in transgenic oilseed rape (Brassica napus) lines and wild plants. The results showed that the three genes were all up-regulated expressed in transgenic lines, which indicated that the mechanism of enhanced resistance to Sclerotinia sclerotiorum of transgenic lines was related to the induction of SA-dependent signaling pathway.

Key words: salicylic acid (SA)-dependent signaling pathway; defense genes; oilseed rape (Brassica napus); Sclerotinia sclerotiorum

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