用于莱茵衣藻荧光定量PCR 分析的内参基因选择

吴文凯1,2, 刘成前2, 周志刚1, 卢山2,*
1 上海海洋大学水产与生命学院, 上海201306; 2 南京大学生命科学学院, 南京210093

通信作者:卢山;E-mail: shanlu@nju.edu.cn;Tel: 025-83594653

摘 要:

本文用GeNorm与NormFinder 算法分析actin、α-tubulin、cblp 和yptc1 四个常用内参基因在不同处理下莱茵衣藻中的表达。结果显示, α-tubulin在各种条件下都有较好的表达稳定性, 而以不同基因组合构成的多内参系统可能更有利于细胞周期短暂的单细胞绿藻的分析。

关键词:莱茵衣藻; 荧光定量PCR; 内参基因

收稿:2009-04-02   修定:2009-06-16

资助:国家自然科学基金(30771167)和上海市教育委员会重点科研项目(科06-411 )。

The Selection of Reference Genes in Chlamydomonas reinhardtii P. A. Dangeard by Real-Time Quantitative PCR

WU Wen-Kai1,2, LIU Cheng-Qian2, ZHOU Zhi-Gang1, LU Shan2,*
1College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; 2School of Life Sciences, Nanjing University, Nanjing 210093, China

Corresponding author: LU Shan; E-mail: shanlu@nju.edu.cn; Tel: 025-83594653

Abstract:

The selection of reference genes plays a key role in the study of gene expression by real-time quantitative PCR. Using GeNorm and NormFinder algorithms, we studied the expression of four common-used reference genes, actin, α-tubulin, cblp and yptc1, in Chlamydomonas reinhardtii under different treatments. Our results showed that the expression of α-tubulin was relatively stable, but it might be more reliable to use a combination of different genes and utilize their normalization factor (NF) as a reference for analyzing the dynamic changing gene expression of unicellular green algae.

Key words: Chlamydomonas reinhardtii; real-time quantitative PCR; reference gene

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