《植物生理学报》 2009, 45(7): 643-646

外源一氧化碳对盐胁迫下水稻悬浮细胞死亡的缓解效应

王智¹, 陈楚琴², 袁星星¹, 徐晟¹, 杨清^1,*, 沈文飚^1,*

南京农业大学1 生命科学学院, 2 食品科技学院, 南京210095

通信作者：杨清；E-mail: qyang19@njau.edu.cn, wbshenh@njau.edu.cn；Tel: 025-84395221;025-84396542

摘　要：

水稻悬浮细胞经 NaCl (0~400 mmol•L-1)处理后, 细胞死亡率随着NaCl浓度的增大和处理时间的延长而上升。50% 饱 和度的外源一氧化碳(CO)溶液预处理 3 h 可以缓解随后由 200 mmol•L-1 NaCl 引起的细胞死亡率上升, CO 清除剂血红蛋白 (Hb)有逆转CO 的保护效应。CO 在不同程度上还可以促进超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物 酶(APX)活性的上升或相应基因的表达。

关键词：盐胁迫; 水稻悬浮细胞; 一氧化碳; 细胞死亡

收稿：2009-04-27   修定：2009-05-27

资助：江苏省环洪泽湖生态农业生物技术重点实验室基金(HZHL0807)和国家自然科学基金(30671248)。

Mitigative Effect of Exogenous Carbon Monoxide on Cell Death in Rice Suspension-Cultured Cells under Salt Stress

WANG Zhi¹, CHEN Chu-Qin², YUAN Xing-Xing¹, XU Sheng¹, YANG Qing^1,*, SHEN Wen-Biao^1,*

¹College of Life Sciences, ²Department of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Corresponding author: YANG Qing; E-mail: qyang19@njau.edu.cn, wbshenh@njau.edu.cn; Tel: 025-84395221;025-84396542

Abstract:

In this study, the role of carbon monoxide (CO) on reducing cell death rate of rice suspensioncultured cells was investigated under salt stess. The cell death rate increased with the increasing of NaCl concentration (0–400 mmol·L^-1) and time (3, 6, 12 and 24 h). Pretreatment with 50% saturation of exogenous CO aqueous solution for 3 h was able to significantly alleviate the elevating cell death rate induced by further 200 mmol·L^-1 NaCl treatment. Meanwhile, the above cytoprotective effect of CO could be reversed by the addition of hemoglobin (Hb), a scavenger of CO. Furthermore, the activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) or their related transcripts were also induced by CO in different degrees.

Key words: salt stress; rice suspension-cultured cells; carbon monoxide; cell death