低温胁迫下玉米幼苗的几种生理生化指标的变化

王小丽, 裴玉贺, 郭新梅, 张恩盈, 宋希云*
青岛农业大学生命科学学院, 山东青岛266109

通信作者:宋希云;E-mail: songxy@qau.edu.cn;Tel: 0532-86080009

摘 要:

胚芽期和三叶期的玉米自交系 ‘ 齐 319’ 幼苗经 0、2、4、6 ℃冷处理后, 测定过氧化物酶(POD)、过氧化氢酶(CAT) 和超氧化物歧化酶(SOD)活性以及可溶性蛋白和丙二醛(MDA)含量的结果表明: POD、CAT、SOD 活性以及 MDA 和蛋白 质含量均增加, 0、2 ℃下胚芽期幼苗的抗寒性比三叶期的高, 4和6 ℃下三叶期幼苗的抗寒能力更强; 无论是胚芽期还是三 叶期, 温度越低, 幼苗受冷伤害越大。

关键词:玉米幼苗; 低温胁迫; 生理生化指标

收稿:2008-11-24   修定:2009-04-20

资助:“ 十一五” 科技支撑计划“ 粮食丰产科技工程”(2006BAD02A09)、山东省农业重大应用技术创新和山东省农业科技成果转化资金项目

Effect of Low Temperature Stress on Some Physiological and Biochemical Indexes of Maize Seedlings

WANG Xiao-Li, PEI Yu-He, GUO Xin-Mei, ZHANG En-Ying, SONG Xi-Yun*
College of Life Sciences, Qingdao Agricultural University, Qingdao, Shandong 266109, China

Corresponding author: SONG Xi-Yun; E-mail: songxy@qau.edu.cn; Tel: 0532-86080009

Abstract:

Maize inbred line ‘Qi319’ seedlings at germ stage and three-leaf stage were treated with 0, 2, 4, 6 ℃, and the activities of peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) and the contents of soluble protein and malondiadehyde (MDA) were determinated. The results showed that the activities of POD, CAT, SOD, and the contents of MDA and soluble protein increased. Under 0 ℃ and 2 ℃, seedlings at germ stage displayed higher cold tolerance than those at three-leaf stage, but under 4 ℃ and 6 ℃, the cold tolerance of three-leaf stage seedlings were stronger than those of germ stage; whether at germ stage or three-leaf stage, the lower the temperature was, the greater seedlings were harmed.

Key words: maize seedlings; low temperature stress; physiological and biochemical indexes

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