血红素加氧酶介导GA 对小麦糊粉层中α- 淀粉酶的诱导表达

武明珠, 徐晟, 崔为体, 张博, 刘雅辉, 凌腾芳, 沈文飚*
南京农业大学生命科学学院, 南京农业大学- 贝克曼离心机合作示范实验室, 南京210095

通信作者:沈文飚;E-mail: wbshenh@njau.edu.cn;Tel: 025-84395773

摘 要:

单独以赤霉素(GA)处理或与HO-1 诱导物高铁血红素(Ht)和CO水溶液组合处理均导致小麦糊粉层中血红素加氧酶(HO)活性的提高, 同时α- 淀粉酶基因表达和α- 淀粉酶活性也明显受诱导; 用HO-1 专一性抑制剂锌原卟啉(ZnPPIX)预处理6 h 后, 上述效应部分受阻断。这暗示HO 可能参与GA诱导的α- 淀粉酶基因表达。

关键词:小麦糊粉层; 血红素加氧酶; 高铁血红素; 一氧化碳; 赤霉素; α-淀粉酶

收稿:2009-03-23   修定:2009-04-15

Involvement of Heme Oxygenase in Gibberellic Acid-induced α-Amylase Expression in Wheat Aleurone Layer

WU Ming-Zhu, XU Sheng, CUI Wei-Ti, ZHANG Bo, LIU Ya-Hui, LING Teng-Fang, SHEN Wen-Biao*
Cooperative Demonstration Laboratory of Centrifuge Technique, Nanjing Agricultural University and Beckman Coulter Ltd. Co.,College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China

Corresponding author: SHEN Wen-Biao; E-mail: wbshenh@njau.edu.cn; Tel: 025-84395773

Abstract:

In this study, we showed that gibberellic acid (GA) with or without HO-1 inducer hematin (Ht) or carbon monoxide (CO) aqueous solution not only led to promote heme oxygenase (HO) activity in wheat aleurone layer, but also enhanced α-amylase gene expression and its enzyme activities. Whereas, application of HO-1 inhibitor zinc protoporphyrin IX (ZnPPIX) for 6 h blocks partially above processes. Together, above results suggested that HO might be involved in GA-induced α-amylase gene expression.

Key words: wheat (Triticum aestivum L.) aleurone layer; heme oxygenase; hematin; carbon monoxide; gibberellic acid; α-amylase

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