PDF1.2 基因在转草酸氧化酶基因甘蓝型油菜中的表达

冀瑞琴1,2, 董祥柏2, 冯辉1, MARTIN Barbetti3, 高荣村2, 刘月英2, 刘胜毅2,*
1 沈阳农业大学园艺学院, 沈阳 110161; 2 中国农业科学院油料作物研究所, 武汉 430062; 3School of Plant Biology, Faculty ofNatural and Agricultural Sciences, the University of Western Australia, Crawley, WA, 60099, Australia

通信作者:刘胜毅;E-mail: liusy@oilcrops.cn;Tel: 027 -86812896

摘 要:

采用RT-PCR 方法检测茉莉酸(JA)/乙烯(ET)依赖性信号途径中关键基因PDF1.2 在转草酸氧化酶基因(OXO)油菜株系与未转化对照中的表达差异。结果表明, 在转基因油菜不同株系中PDF1.2都有不同程度的上调表达, 预示着转OXO油菜对菌核病的抗性增强可能与激活JA/ET依赖性信号途径有关

关键词:甘蓝型油菜; 菌核病; PDF1.2

收稿:2009-03-09   修定:2009-04-28

资助:国家自然科学基金(30671344、30800748)和中国博士后科学基金(20070411080)。

Expression of PDF1.2 in Oxalate Oxidase Transgenic Oilseed Rape (Brassica napus L.)

JI Rui-Qin1,2, DONG Xiang-Bai2, FENG Hui1, MARTIN Barbetti3, GAO Rong-Cun2, LIU Yue-Ying2, LIU Sheng-Yi2,*
1College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China;2Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; 3School of Plant Biology, Faculty of Natural and Agricultural Sciences, the University of Western Australia, Crawley, WA, 6009, Australia

Corresponding author: LIU Sheng-Yi; E-mail: liusy@oilcrops.cn; Tel: 027 -86812896

Abstract:

The expressions of PDF1.2, a key gene in the jasmonic acid (JA)/ethylene (ET)-dependent signaling pathway, were measured in oxalate oxidase (OXO) transgenic and non-transgenic oilseed rapes (Brassica napus) by RT-PCR. The results showed that the expression of PDF1.2 was up-regulated in different OXO transgenic oilseed rape lines, which indicated that the mechanism of disease resistance to Sclerotinia sclerotiorum was related to the induction of the JA/ET-dependent signaling pathway.

Key words: oilseed rape (Brassica napus); Sclerotinia sclerotiorum; PDF1.2

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