外源一氧化氮供体硝普钠对干旱胁迫下小麦幼苗叶中 ATP 酶活性和膜脂过氧化的影响

李慧1, 赵文才1, 赵会杰2, 韩燕来1, 谭金芳1,*
河南农业大学1 资源环境学院, 2 生命科学学院, 郑州450002

通信作者:谭金芳;E-mail: tanjf@henau.edu.cn;Tel: 0371-63554088

摘 要:

研究外源一氧化氮(NO)供体硝普钠(SNP)对干旱胁迫下小麦幼苗叶片ATP酶活性和膜脂过氧化影响的结果表明, 15%聚乙二醇-6000 (PEG-6000)模拟的干旱胁迫下小麦幼苗叶中H+-ATP 酶和Ca2+-ATP 酶活性显著升高后迅速下降, 硫代巴比妥酸反应产物(TBARS)和质量膜透性增加; 0.1 mmol·L-1 SNP可提高干旱胁迫下小麦幼苗叶中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性, 降低超氧阴离子(O2-·)和过氧化氢(H2O2)水平, 缓解膜脂过氧化, 稳定生物膜的结构和功能, H+-ATP 酶和Ca2+-ATP 酶也可以保持更高的活性。

关键词:一氧化氮; 干旱胁迫; 小麦幼苗叶片; ATP酶活性; 膜脂过氧化

收稿:2009-01-15   修定:2009-04-20

资助:国际植物营养研究所(IPNI)项目(30200108)、“ 十一五”国家科技支撑计划(30200166)和河南省农业科技成果转化基金项目(05360000006)。

Effects of Exogenous Nitric Oxide Donor Sodium Nitroprusside on ATPase Activity and Membrane Lipid Peroxidation in Wheat (Triticum aestivum L. cv.‘Luohan 6’) Seedling Leaves under Drought Stress

LI Hui1, ZHAO Wen-Cai1, ZHAO Hui-Jie2, HAN Yan-Lai1, TAN Jin-Fang1,*
1College of Environmental and Resource Sciences, 2College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China

Corresponding author: TAN Jin-Fang; E-mail: tanjf@henau.edu.cn; Tel: 0371-63554088

Abstract:

The effects of nitric oxide (NO) donor sodium nitroprusside (SNP) on ATPase activity and membrane lipid peroxidation in the wheat (Triticum aestivum cv. ‘Luohan 6’) seedling leaves under drought stress were investigated. Results showed that the 15% PEG-6000 treatment apparently enhanced the H +-ATPase andCa2+-ATPase activities before period of deterioration, accelerated the accumulation of thiobarbituric acid reactive substances (TBARS) and membrane permeability in wheat seedling leaves under drought stress. 0.1 mmol·L-1SNP could enhance the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), lower O2¯ production rate, H2O2 content and membrane lipid peroxidation, stabilize the structure and function of biomembranes, increase the activities H+-ATPase and Ca2+-ATPase.

Key words: nitric oxide; drought stress; wheat seedling leaves; ATPase activity; membrane lipid peroxidation

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