光下叶绿素缺乏的大麦突变体NYB 中叶绿素合成再探

田文娟, 袁明, 陈洋尔, 张中伟, 徐飞, 袁澍, 林宏辉*
四川大学生命科学学院, 成都610064

通信作者:林宏辉;E-mail: honghuilin@vip.sina.com;Tel: 028-85415389

摘 要:

研究一种原叶绿素酯还原酶(POR)的大麦突变体NYB光下合成叶绿素的结果表明, NYB中PORB的含量比野生型低。 NYB 前质体中原片层体的大小和数量与野生型差不多, 但其结构比野生型的松散。暗中生长的突变体内POR 蛋白复合物 LHPP比野生型少。不同光照强度下叶绿素积累的结果显示, 光照度越强, 突变体与野生型的叶绿素差异越显著。由于porB 是单拷贝的, 所以推测突变体中部分porB mRNA可能产生错误的剪切拼接, 以致光下突变体NYB仍然能合成叶绿素。

关键词:叶绿素; 黄化大麦; POR; LHPP

收稿:2008-11-20   修定:2009-02-21

资助:国家重点基础研究发展计划(973计划) 项目(2009CB118500)、国家自然科学基金(30571119 和30800071)和教育部重点项目(108110 和NCET-05-0786)。

Further Study on Chlorophyll Synthesis of a Chlorophyll-Less Barley Mutant NYB in Light

TIAN Wen-Juan, YUAN Ming, CHEN Yang-Er, ZHANG Zhong-Wei, XU Fei, YUAN Shu, LIN Hong-Hui*
College of Life Science, Sichuan University, Chengdu 610064, China

Corresponding author: LIN Hong-Hui; E-mail: honghuilin@vip.sina.com; Tel: 028-85415389

Abstract:

The chlorophyll synthesis of a protochlorophyllide oxidoreductase (POR) in barley mutant NYB was studied, and we found that the content of PORB was less in NYB than that in the wild type (WT). In the etioplasts of NYB, prolamellar bodies had similar size and amount with WT, but had a more incompact structure. The content of POR complex LHPP also was much less in the dark-grown mutant than that in WT. The data about the accumulation of chlorophyll in different intensities of light demonstrated that the stronger the illumination was, the more prominent difference of chlorophylls between the mutant and WT was. As the porB gene is a single copy, we extrapolated that probably partial porB mRNAs had wrong cutting and splicing, and therefore the mutant NYB could synthesize chlorophyll in the light.

Key words: chlorophyll; yellowish barley; POR; LHPP

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