玻璃化法超低温保存蛇莓茎尖

薄涛, 王子成*, 柴星星
河南大学生命科学学院, 河南开封 475004

通信作者:王子成;E-mail: wzc@henu.edu.cn;Tel: 0378-2868833-3767

摘 要:

本文采用玻璃化法对蛇莓离体茎尖超低温保存进行了初步探讨。研究了低温锻炼时间、预培养时间、预处理时间、玻璃化液处理时间和液氮保存时间对超低温保存后成活率的影响。经优化, 蛇莓的最高成活率可达(42.00±2.74)%

关键词:蛇莓; 茎尖; 超低温保存; 玻璃化; 成活率

收稿:2010-05-21   修定:2010-06-18

资助:国家自然科学基金(30900973)。

Cryopreservation of Duchesnea indica (Andr.) Focke Shoot-Tips by Vitrification

BO Tao, WANG Zi-Cheng*, CHAI Xing-Xing
College of Life Science, Henan University, Kaifeng, Henan 475004, China

Corresponding author: WANG Zi-Cheng; E-mail: wzc@henu.edu.cn; Tel: 0378-2868833-3767

Abstract:

A procedure had been studied on cryopreservation of Duchesnea indica (Andr.) Focke shoot-tips in vitro by vitrification. The influence of the treatment periods of cold temperature acclimation, pre-culture, pretreatment, PVS2 and cryopreservation in liquid nitrogen on survival rate after cryopreservation was studied. The best survival rate reached to (42.00±2.74)%.

Key words: Duchesnea indica; shoot-tip; cryopreservation; vitrification; survival rate

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