马铃薯StAP1 基因的分子克隆与表达分析

樊春媛1, 印敬明1, 王冰1, 韦青1, 李根涛1, 张云峰1,2 , 杨清1,*
1 南京农业大学生命科学学院, 作物遗传与种质创新国家重点实验室, 南京210095; 2 江苏省环洪泽湖生态农业生物技术重点实验室, 江苏淮安 223300

通信作者:杨清;E-mail: qyang19@njau.edu.cn;Tel: 025-84395221

摘 要:

采用 RT-PCR 方法从马铃薯品种 ‘Désirée’ 中克隆了 StAP1 cDNA 序列(GenBank 登录号 GU220568)。该基因 cDNA 开放阅读框长度为735 bp, 编码一个由244个氨基酸残基组成的蛋白, 该蛋白分子量为28.57 kDa, 理论等电点为8.32。StAP1 蛋白含有 1 个高度保守的 MADS 结构域, 与烟草 NAP1 具有较高一致性。组织表达分析显示, StAP1 在马铃薯植株的顶芽、 花和叶中有较高水平的转录表达, 在块茎中有微量表达。利用反义 StCOL 转基因马铃薯植株进一步分析表明, StAP1 的表 达受 StCOL 的调控。

关键词:马铃薯; StAP1; 克隆; 表达

收稿:2010-04-01   修定:2010-06-12

资助:江苏省环洪泽湖生态农业生物技术重点实验室开放基金(HZHL0807)和作物遗传与种质创新国家重点实验室开放基金(ZW2007003)。

Molecular Cloning and Expression Analysis of a Potato StAP1 Gene

FAN Chun-Yuan1, YIN Jing-Ming1, WANG Bing1, WEI Qing1, LI Gen-Tao1, ZHANG Yun-Feng1,2, YANG Qing1,*
1State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China; 2Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaian, Jiangsu 223300, China

Corresponding author: YANG Qing; E-mail: qyang19@njau.edu.cn; Tel: 025-84395221

Abstract:

A homologue of APETALA1 gene, designated StAP1, was isolated from potato ‘Désirée’ (Solanum tuberosum) by RT-PCR (GenBank accession number GU220568). The ORF of the StAP1 was 735 bp long and encoded a putative protein of 244 amino acids with a molecular weight of 28.57 kDa and a theoretical pI of 8.32. StAP1 protein contained a conserved MADS domain and had a high identity with AP1 homologous member from tobacco. Analysis on the mRNA level of StAP1 showed that it was highly expressed in leaves, apical buds and flowers, and was expressed at lower levels in tubers. Further analysis with StCOL-antisense transgenic potato plants indicated that StAP1 expression was regulated by StCOL.

Key words: potato; StAP1; cloning; expression

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