不同培养模式及生物膜含水量对栅藻生长和油脂积累的影响


刘颖颖,朱梅,崔红利,张春辉,孙希平*,季春丽*,李润植
山西农业大学农学院/分子农业与生物能源研究所, 山西晋中030801

通信作者:孙希平;E-mail: sxpljj@126.com;季春丽;E-mail: jichunli@sxau.edu.cn

摘 要:

微藻生物膜培养技术与传统液体悬浮培养方法相比具有显著增加生物量产率的优势, 但关于微藻生物膜培养模式下由于大水体减少对藻细胞生理变化造成的影响还尚不清楚。为了对比悬浮培养和吸附式生物膜培养, 以及不同生物膜含水量下栅藻(Scenedesmus dimorphus)的生长、油脂积累以及光合活性的变化, 本研究将栅藻接种于微孔滤膜上并置于BG11固体培养基上, 通过向培养基中添加不同浓度的琼脂来控制生物膜含水量, 并以液体悬浮培养作为对照研究其生理变化。结果表明, 随着培养基中琼脂浓度的升高, 栅藻生物膜含水量以及生物膜相对含水量均逐渐降低。而生物膜培养7 d后的栅藻生物量为65.79~85.28 g·m−2, 均高于液体悬浮培养的36.40 g·m−2; 并且生物膜培养的生物量产率最高达到10.78g·m−2·d−1, 远远高于悬浮培养的3.80 g·m−2·d−1, 同时生物膜培养栅藻的油脂产率为1.93~2.70 g·m−2·d−1, 均高于对照悬浮培养0.73 g·m−2·d−1。生物膜培养栅藻的色素含量在高生物膜含水量下与悬浮培养相似, 但低生物膜含水量下低于悬浮培养。结合色素含量和叶绿素荧光参数总体来看, 生物膜培养栅藻的光合能力不如液体悬浮培养, 但生物膜培养避免了光在液体中的耗散, 使更多的细胞可得到有效光照, 从而整体表现出高的光合效率与生物量产率。


关键词: 栅藻; 生物膜培养; 生长; 油脂; 光合特性

收稿:2021-06-18   修定:2021-10-22

资助:国家自然科学基金(31902344)、山西省高等学校科技创新项目(2019L0376)和山西农业大学科技创新基金项目(2016YJ01)。

Effect of cultivation mode and biofilm moisture content on the growth and lipid accumulation of Scenedesmus dimorphus

LIU Yingying, ZHU Mei, CUI Hongli, ZHANG Chunhui, Sun Xiping*, JI Chunli*, LI Runzhi
Institute of Molecular Agriculture & Bioenergy, College of Agriculture, Shanxi Agricultural University, Jinzhong, Shanxi 030801, China

Corresponding author: Sun Xiping; E-mail: sxpljj@126.com; JI Chunli; E-mail: jichunli@sxau.edu.cn

Abstract:

Microalgae biofilm cultivation technology has the advantage of significantly improving the biomass 

productivity compared with the conventional aqua-suspended cultivation method. However, it is still unclear about the physiological changes of microalgae cells due to the reduced water body under the biofilm cultivation. In order to compare the growth, lipid accumulation and photosynthetic activity of Scenedesmus

dimorphus in suspended and biofilm cultivation, as well as under different biofilm moisture contents, algal cells was inoculated on the filtration membrane and placed on BG11 agar solid medium (biofilm cultivation) with inoculation in BG11 liquid medium (suspended cultivation) as control. Meanwhile, biofilm moisture content was controlled by adding different concentrations of agar to the medium in biofilm cultivation. The results showed that with the increase of agar concentration in the medium, both the moisture content and relative moisture content of biofilm were gradually decreased. The biomass density of S. dimorphus under biofilm cultivation after 7 days growth was in the range of 65.79–85.28 g·m–2, which was higher than that under suspended cultivation (36.40 g·m–2). And the maximum biomass productivity of biofilm cultivation was 10.78 g·m–2·d–1, which was much higher than that of 3.80 g·m–2·d–1 in suspended cultivation. The lipid productivity of S. dimorphus in biofilm cultivation ranged from 1.93 to 2.70 g·m–2·d–1 while it was 0.73 g·m–2·d–1 in suspended cultivation. The pigment content of S. dimorphus in biofilm cultivation especially under low biofilm moisture content was lower than that in suspended cultivation. Combined with pigment content and chlorophyll fluorescence parameters, although the photosynthetic capacity of biofilm cultured S. dimorphus was not as good as in liquid suspended cultivation, the light dissipation in liquid was avoided in biofilm cultivation and more cells could receive effective light so that biofilm cultivation showed overall high photosynthetic efficiency and biomass productivity.


Key words: Scenedesmus dimorphus; biofilm cultivation; growth; lipid; photosynthetic characteristics

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