桔梗萜类合成途径PgMCS和PgHDR基因克隆与原核表达分析

李景1,刘梦丽1,余函纹1,周卓1,常相伟1,王举涛1,查良平1,2,*,桂双英1,3,4,*
1安徽中医药大学药学院, 合肥230012;2安徽省中医药科学院中药资源保护与开发研究所, 合肥230012;3安徽省中医药科学院药物制剂研究所, 合肥230012;4安徽省教育厅现代药物制剂工程技术研究中心, 合肥230012

通信作者:查良平;E-mail: zlp_ahtcm@126.com;桂双英;E-mail: guishy0520@126.com

摘 要:

关键词:桔梗; MCS; HDR; 基因克隆; 原核表达

收稿:2021-10-18   修定:2021-12-17

资助:国家自然科学基金区域联合基金项目(U21A20406)、安徽省中央引导地方科技发展专项资金(YDZX20183400004233)、中央本级 重大增减支项目(2060302)和2020年安徽高校合作攻关和公共卫生协同创新项目(GXXT-2020-025)。

Gene cloning and prokaryotic expression analysis for the PgMCS and PgHDR genes from the Platycodon grandiflorum terpenoid biosynthesis pathway

LI Jing1, LIU Mengli1, YU Hanwen1, ZHOU Zhuo1, CHANG Xiangwei1, CHANG Xiangwei1, ZHA Liangping1,2,*, GUI Shuangying1,3,4,*
11School of Pharmacy, Anhui University of Chinese Medicine (AUCM), Hefei 230012, China; 22Institute of Conservation and Development of Traditional Chinese Medicine Resources, Anhui Academy of Chinese Medicine, Hefei 230012, China; 3Institute of Pharmaceutics, Anhui Academy of Chinese Medicine, Hefei 230012, China; 4Engineering Technology Research Center of Modernized Pharmaceutics, Anhui Education Department (AUCM), Hefei 230012, China

Corresponding author: ZHA Liangping; E-mail: zlp_ahtcm@126.com; GUI Shuangying; E-mail: guishy0520@126.com

Abstract:

Key words: Platycodon grandiflorum; MCS; HDR; gene cloning; prokaryotic expression

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