粉葛叶片愈伤组织诱导及植株再生

曾文丹,严华兵*,肖亮,尚小红,曹升,陆柳英,赖大欣
广西壮族自治区农业科学院经济作物研究所, 南宁530007

通信作者:严华兵;E-mail: h.b.yan@hotmail.com

摘 要:

以广西粉葛品种桂粉葛一号组培苗叶片为外植体, 探讨不同植物生长调节剂、叶片取材部位、培养条件等对愈伤组织诱导及不定芽发生的影响。结果表明: 2~5节位完全展开的幼嫩叶为外植体材料,暗培养21 d为愈伤组织诱导的最适培养条件; 在仅含生长素的培养基中, 粉葛愈伤组织诱导率低, 且形成的愈伤组织无分化能力; 愈伤组织诱导最适培养基为MS+2.0 mg·L-1 6-苄基腺嘌呤(6-benzylaminopurine, 6-BA)+1.0 mg·L-1 2,4-二氯苯氧乙酸(2,4-dichlorophenoxy acetic acid, 2,4-D), 诱导率为94.7%; 愈伤组织分化不定芽的最适培养基为MS+2.0 mg·L-1 6-BA+0.1 mg·L-1萘乙酸(naphthalene acetic acid, NAA), 分化率为9.2%。将诱导分化的不定芽转入MS+0.02 mg·L-1 NAA培养基中生根率达98%以上; 将生长良好的再生植株进行移栽驯化, 存活率达96.7%

关键词:粉葛; 组培苗叶片; 愈伤组织; 不定芽; 植株再生

收稿:2021-02-07   修定:2021-04-20

资助:国家自然科学基金(31960420)、广西自然科学基金(2020GXNSFAA297187)、广西重点研发计划(桂科AB1850028)和广西农业科 学院基本科研业务专项(桂农科2021JM64和桂农科2021YT057)

Callus induction and plant regeneration from leaves of Pueraria thomsonii

ZENG Wendan, YAN Huabing*, XIAO Liang, SHANG Xiaohong, CAO Sheng, LU Liuying, LAI Daxin
Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China

Corresponding author: YAN Huabing; E-mail: h.b.yan@hotmail.com

Abstract:

The plantlet leaves of Guangxi variety Pueraria thomsonii ‘Guifenge No. 1’ were used as explants
to study the effects of different plant growth regulators, sampling position of leaves and culture conditions on callus induction and adventitious buds formation. The results showed that the best explants were fully expanded young leaves at 2–5 nodes and the best condition for callus induction were dark culture for 21 days. In the MS medium containing auxin only, the callus induction rate was low, and the formed calli had no differentiation ability. The optimal callus induction medium was MS + 2.0 mg·L
-1 6-BA + 1.0 mg·L-1 2,4-D, whose induction rate reached 94.7%. The optimal medium for adventitious buds induction was MS + 2.0 mg·L-1 6-BA + 0.1 mg·L-1 NAA, and callus differentiation rate was 9.2%. When the adventitious buds were transferred into rooting medium MS + 0.02 mg·L-1 NAA, the rooting rate was more than 98%. The regenerated plants with good growth were transplanted and domesticated, and the survival rate reached 96.7%.

Key words: Pueraria thomsonii; plantlet leaves; callus; adventitious bud; plant regeneration

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