《植物生理学报》 2021, 57(5): 1098-1104
以广西粉葛品种‘桂粉葛一号’组培苗叶片为外植体, 探讨不同植物生长调节剂、叶片取材部位、培养条件等对愈伤组织诱导及不定芽发生的影响。结果表明: 以2~5节位完全展开的幼嫩叶为外植体材料,暗培养21 d为愈伤组织诱导的最适培养条件; 在仅含生长素的培养基中, 粉葛愈伤组织诱导率低, 且形成的愈伤组织无分化能力; 愈伤组织诱导最适培养基为MS+2.0 mg·L-1 6-苄基腺嘌呤(6-benzylaminopurine, 6-BA)+1.0 mg·L-1 2,4-二氯苯氧乙酸(2,4-dichlorophenoxy acetic acid, 2,4-D), 诱导率为94.7%; 愈伤组织分化不定芽的最适培养基为MS+2.0 mg·L-1 6-BA+0.1 mg·L-1萘乙酸(naphthalene acetic acid, NAA), 分化率为9.2%。将诱导分化的不定芽转入MS+0.02 mg·L-1 NAA培养基中生根率达98%以上; 将生长良好的再生植株进行移栽驯化, 存活率达96.7%。
关键词：粉葛; 组培苗叶片; 愈伤组织; 不定芽; 植株再生
Corresponding author: YAN Huabing; E-mail: firstname.lastname@example.org
The plantlet leaves of Guangxi variety Pueraria thomsonii ‘Guifenge No. 1’ were used as explants
to study the effects of different plant growth regulators, sampling position of leaves and culture conditions on callus induction and adventitious buds formation. The results showed that the best explants were fully expanded young leaves at 2–5 nodes and the best condition for callus induction were dark culture for 21 days. In the MS medium containing auxin only, the callus induction rate was low, and the formed calli had no differentiation ability. The optimal callus induction medium was MS + 2.0 mg·L-1 6-BA + 1.0 mg·L-1 2,4-D, whose induction rate reached 94.7%. The optimal medium for adventitious buds induction was MS + 2.0 mg·L-1 6-BA + 0.1 mg·L-1 NAA, and callus differentiation rate was 9.2%. When the adventitious buds were transferred into rooting medium MS + 0.02 mg·L-1 NAA, the rooting rate was more than 98%. The regenerated plants with good growth were transplanted and domesticated, and the survival rate reached 96.7%.
Key words: Pueraria thomsonii; plantlet leaves; callus; adventitious bud; plant regeneration
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