木瓜转录组数据SSR标记的开发及其遗传多样性分析

伍越1,4,王甜甜1,2,李泽秀1,纪庆柱1,毛培利1,诸葛绪钦3,曹帮华1,*
1山东农业大学林学院, 山东泰安271018;2东营市园林绿化中心, 山东东营257091;3临沂市多维木瓜饮品有限公司, 山东临沂276026;4北京林木分子设计育种高精尖创新中心/林木育种国家工程实验室/北京林业大学生物科学与技术学院, 北京 100083

通信作者:曹帮华;E-mail: caobh@sdau.edu.cn

摘 要:

试验对木瓜属(Chaenomeles) 58份种质进行遗传多样性研究, 为木瓜种质资源鉴定与遗传改良提供了参考。基于皱皮木瓜长俊和光皮木瓜豆青转录组测序数据进行简单重复序列标记(SSR)位点检测,利用毛细管电泳法进行荧光SSR-PCR产物检测。结果表明: 共筛选出22EST-SSR多态性标记, 58份木瓜资源中扩增检测出平均多态性条带(NPB) 6.95个、等位基因数(Na)5.64、有效等位基因数(Ne)3.76Shannon’s信息指数(I)1.395 5Nei’s基因多样性指数(H)0.840 4和多态性信息含量(PIC)0.632 7。群体结构、聚类及主成分分析表明, 58份木瓜种质划分为5个亚群, 遗传相似系数在0.58~0.95之间, 光皮木瓜、日本木瓜显著区分, 毛叶木瓜和西藏木瓜的亲缘关系最近, 与皱皮木瓜的亲缘关系次之。

关键词:木瓜; 转录组; SSR; 遗传多样性

收稿:2020-11-02   修定:2021-03-22

资助:山东省林业科技创新项目(2019LY00104和LYCX02-2018-12)、山东省重大科技创新工程(2017CXGC0316)和山东省农业应用技术 创新课题“良种木瓜高产优质和加工利用提升技术”。

Identifcation of Chaenomeles EST-SSR markers and phylogenetic analysis

WU Yue1,4, WANG Tiantian1,2, LI Zexiu1, JI Qingzhu1, MAO Peili1, ZHUGE Xuqin3, CAO Banghua1,*
1College of Forestry, Shandong Agricultural University, Taian, Shandong 271018, China; 2Dongying Landscape and Greening Department, Dongying, Shandong 257091, China; 33Linyi Multivitamin Chaenomeles Speciosa Beverage GO. LTD, Linyi, Shandong 276026, China; 4Beijing Advanced Innovation Center for Tree Breeding by Molecular Design/National Engineering Laboratory for Tree Breeding/College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China

Corresponding author: CAO Banghua; E-mail: caobh@sdau.edu.cn

Abstract:

In this experiment, 58 germplasms of Chaenomeles were studied for genetic diversity, which provided references for identifcation and genetic improvement of Chaenomeles germplasm resources. SSR locus detection was carried out based on the transcriptome sequencing data of Chaenomeles speciosa ‘Changjun’ and Chaenomeles sinensis ‘Douqing’, and fluorescent SSR-PCR products were detected by capillary electrophoresis. The results showed that 22 pairs of EST-SSR polymorphic markers were screened out. The average polymorphic band (NPB) was 6.95, the allele number (Na) was 5.64, the effective allele number (Ne) was 3.76, Shannon's information index (I) was 1.395 5, Nei's gene diversity index (H) was 0.840 4 and polymorphism information content (PIC) was 0.632 7. The results of population structure, cluster and principal component analysis showed that 58 Chaenomeles germplasm were divided into 5
categories, and the genetic similarity coefficient was between 0.58-0.95.
Chaenomeles japonica and
Chaenomeles sinensis were obviously distinguished from other materials, and the kinship between
Chaenomeles cathayensis and Chaenomeles thibetica is the closest, followed by that of Chaenomeles speciosa.

Key words: Chaenomeles; transcriptome; SSR; genetic diversity

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