萱草花粉电击转化参数的选择

钟荣,谭雨馨,张铮,尹冬梅,刘翔,秦巧平,张志国,倪迪安*
上海应用技术大学生态技术与工程学院, 上海201418

通信作者:倪迪安;E-mail: dani@sit.edu.cn

摘 要:

以萱草品种斯特拉成熟花粉为材料, 比较了不同电场强度和电击次数对花粉转化率的影响。结果显示: 萱草花粉电击转化的最佳参数为1 kV·cm–1、电击3次。

关键词:萱草; 花粉; 电击转化

收稿:2020-11-28   修定:2021-01-27

资助:上海市科技兴农项目[沪农科推字(2019)第1-8号]

Selection of pollen electroporation transformation parameters of daylily (Hemerocallis sp.)

ZHONG Rong, TAN Yuxin, ZHANG Zheng, YIN Dongmei, LIU Xiang, QIN Qiaoping, ZHANG Zhiguo, NI Di’an*
School of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, China

Corresponding author: NI Di’an; E-mail: dani@sit.edu.cn

Abstract:

Different pulse feld strength and number of pulses were used for electroporation transformation of mature pollens of daylily (Hemerocallis sp.) ‘Black Eyed Stella’ to obtain an optimal parameter. The results showed that the optimal parameter of daylily pollen electroporation was 1 kV·cm–1 with 3 pulses

Key words: daylily (Hemerocallis sp.); pollen; electroporation transformation

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