发根农杆菌介导白羽扇豆转基因过表达体系的建立

詹玉洁,刘博文,张仟,许卫锋,夏天雨*
福建农林大学生命科学学院, 福州350002

通信作者:夏天雨;E-mail: tyxia@fafu.edu.cn

摘 要:

白羽扇豆(Lupinus albus)是研究耐低磷机制的模式作物, 然而遗传转化体系的缺乏极大限制了其功能基因组学研究。本试验以白羽扇豆品种‘Amiga’为材料, 研究不同发根农杆菌菌株对白羽扇豆毛状根诱导率和阳性转化率的影响。结果发现K599为白羽扇豆发根遗传转化的最适菌种, 进而确立了一套高效的白羽扇豆毛状根转化体系, 并成功获得增强型绿色荧光蛋白基因EGFP (ENHANCED GREEN FLUORESCENT PROTEIN)在白羽扇豆毛状根中过表达的嵌合植株。该遗传转化体系的建立为白羽扇豆功能基因组学研究提供了强有力的方法支撑。

关键词:白羽扇豆; 发根农杆菌; 毛状根; 遗传转化; 嵌合植株

收稿:2020-09-23   修定:2021-01-28

资助:国家重点研发计划(2017YFE0118100、 2018YFD02003025) 和国家自然科学基金(31872169)

Establishment of Agrobacterium rhizogenes-mediated gene overexpression system in white lupin

ZHAN Yujie, LIU Bowen, ZHANG Qian, XU Weifeng, XIA Tianyu*
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Corresponding author: XIA Tianyu; E-mail: tyxia@fafu.edu.cn

Abstract:

White lupin (Lupinus albus) has become an important model for the study of plant adaptation to phosphorus defciency. However, the lack of Agrobacterium-mediated transformation has limited the functional genomic research extensively. Here, we have used white lupin cultivar ‘Amiga’ as the material to investigate the effects of Agrobacterium rhizogenes strains on induction and positive transformation rates of white lupin hairy roots. The result showed that K599 was the optimal strain for white lupin hairy root transformation. We then established a highly efcient Agrobacterium rhizogenes-based hairy root transformation system of white lupin and obtained a composite plant comprising the wild-type shoot and the EGFP (ENHANCED GREEN FLUORESCENT PROTEIN) overexpression hairy root. This protocol provides a powerful method for functional genomics of white lupin.

Key words: white lupin; Agrobacterium rhizogenes; hairy root; genetic transformation; chimeric plant

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