苹果MdMYB113基因响应高盐胁迫的功能鉴定

李星伟,王庆江,吴宇童,梁伟玲,吴瑞刚*,程福厚*
河北工程大学园林与生态工程学院, 河北邯郸056038

通信作者:吴瑞刚;E-mail: wuruigang1986@126.com;程福厚;E-mail: 13831037138@163.com

摘 要:

MYB转录因子家族作为植物中主要的转录因子家族之一, 在响应非生物胁迫中发挥重要的调节作用。本研究以苹果金冠为试验材料, 基于苹果基因组数据库克隆了MYB转录因子家族基因MdMYB113。该基因编码区全长为804 bp, 可编码267个氨基酸, 等电点为5.12。系统进化树结果表明MdMYB113与拟南芥的AtMYB13/14/15亲缘关系较近。氨基酸序列比对分析显示, MdMYB113蛋白具有R2R3序列的保守结构域, 属于R2R3类型的MYB转录因子。 MdMYB113蛋白定位于细胞核, 具有一定的转录自激活活性。定量表达分析表明MdMYB113基因会因高盐胁迫显著诱导而上调表达。在苹果愈伤中过表达MdMYB113增强了其对盐胁迫的耐受性。综上所述, 证明MdMYB113可能作为正调控因子参与到高盐胁迫反应中。

关键词:MdMYB113基因; 转录因子; 盐胁迫; 功能鉴定

收稿:2020-11-11   修定:2021-02-01

资助:河北省青年科学基金(C2019402255)、河北工程大学博士专项基金(SJ010002101)

Functional identifcation of MdMYB113 gene in apple in response to high salt stress

LI Xingwei, WANG Qingjiang, WU Yutong, LIANG Weiling, WU Ruigang*, CHENG Fuhou*
School of Landscape and Ecological Engineering, Hebei University of Engineering, Handan, Hebei 056038, China

Corresponding author: WU Ruigang; E-mail: wuruigang1986@126.com; CHENG Fuhou; E-mail: 13831037138@163.com

Abstract:

MYB transcription factor family, acting as one of the main transcription factor families in plants, plays an important regulatory role in response to abiotic stress. In this study, Malus domestica ‘Golden Delicious’ was taken as the test material, and the MYB transcription factor gene MdMYB113 was cloned based on the apple genome database. The coding region of the gene is 804 bp, encoding 267 amino acids with the isoelectric point of 5.12. The phylogenetic tree results showed that MdMYB113 was closely related to AtMYB13/14/15 in Arabidopsis. Amino acid sequence comparison analysis showed that MdMYB113 protein had conserved domains of R2 and R3 sequences, and belonged to the MYB transcription factor of R2R3 type. MdMYB113 protein was located in the nucleus and had certain transcriptional auto-activation activity. Quantitative expression analysis showed that the expression of MdMYB113 gene would be signifcantly up-regulated due to strong induction by high salt stress. The over-expression of MdMYB113 in transgenic apple callus enhanced its tolerance to salt stress. In summary, it is proved that the MdMYB113 may be involved in the high salt stress response as a positive regulator.

Key words: MdMYB113 gene; transcription factor; salt stress; functional identifcation

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