《植物生理学报》 2021, 57(2): 501-511
以西藏黄牡丹花粉为材料, 采用扫描电镜观察了花粉表观特征, 利用离体培养法研究花粉的萌发特性, 并探讨了不同贮存温度及时间对花粉寿命、 SOD、 POD、 CAT活性、 MDA和AsA含量的影响。结果表明: 黄牡丹林芝种群花粉大小约为51.35 μm×3.85 μm, 表面纹饰为粗网筛状。花粉饱满率是影响花粉萌发率的主要因素。影响西藏黄牡丹花粉萌发的因子依次为: 蔗糖>硼酸>Ca(NO3)2>GA3, 最适宜花粉萌发的培养基为: 120 g·L-1蔗糖+45 mg·L-1硼酸+30 mg·L-1 Ca(NO3)2+55 mg·L-1 GA3。不同贮藏温度和贮藏时间对花粉发芽率的影响有显著差异, 花粉贮存的最佳温度为–196°C。花粉萌发率与3种保护酶活性、 AsA含量呈显著正相关, 与MDA含量呈显著负相关。室温下POD为敏感性保护酶, 4°C下SOD和POD是敏感性保护酶, –20和–80°C下, SOD为敏感性保护酶; 3种保护酶活性及AsA含量对花粉萌发率的影响次序为: SOD>CAT>AsA>POD。花粉贮存期间花粉保护酶活性、 MDA和AsA含量保持稳定是花粉保持高活力的生理特征。
关键词：西藏黄牡丹; 花粉; 寿命; 贮存; 保护酶; 丙二醛; 抗坏血酸
Corresponding author: JIA Wenqing; E-mail: email@example.com
The pollen of Paeonia lutea was used to observe the pollen morphology by scanning electron microscope. The germination characteristics of pollen were investigated by in vitro culture method. The effect of different storage temperature and time on pollen longevity, SOD, POD, CAT activities, MDA and AsA contents were explored. The results showed that the pollen size of P. lutea Linzhi population was about 51.35 μm×3.85 μm, and pollen ornamentation was rough reticulate. The pollen fullness was the main factor affecting the pollen germination rate. And the factors affecting the germination of P. lutea pollen was: sucrose>boric acid>Ca(NO3)2>GA3. The optimal medium for pollen germination was 120 g·L–1 sucrose+45 mg·L–1 boric acid+30 mg·L–1 Ca(NO3)2+55 mg·L–1 GA3. The effects of different storage temperature and time on pollen germination rate varied signifcantly, and the best storage temperature was -196°C. The pollen germination rate were signifcantly positively correlated with the activities of three protective enzymes, AsA content, and signifcantly negatively correlated with MDA content. At room temperature POD served as a sensitive protective enzyme, SOD and POD served as sensitive protective enzymes at 4°C, and SOD was sensitive protective enzyme at -20°C and -80°C. The order of the effects of three protective enzymes activities and AsA content on the germination rate of pollen was: SOD>CAT>AsA>POD. During pollen storage, the stability of pollen protective enzyme activities, MDA content and AsA content were the physiological characteristics of maintaining high pollen activity.
Key words: Paeonia lutea; pollen; longevity; storage; protective enzymes; MDA; AsA
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