培养基的杨氏模量变化对拟南芥根系生长发育的影响

孟润1,2,王伯初1,2,*
1重庆大学生物工程学院, 重庆400044;2重庆大学生物流变科学与技术教育部重点实验室, 重庆400044

通信作者:王伯初;E-mail: wangbc2000@126.com

摘 要:

力学环境对植物根系生长发育及形态结构的影响受到国内外研究者越来越多的关注, 但是目前相关研究着重于力学刺激下根系的生理学变化, 对定量力学调控根系生长发育的研究仍然缺乏。同时, 土壤紧实程度的力学刺激会直接影响根系发育, 进而会对农作物生长发育和其产量造成影响。因此, 建立机械强度可控的培养基模型来模拟土壤条件, 这对于研究根系在土壤中的穿透生长情况具有重要意义。本研究基于Phytagel培养基成功构建了梯度杨氏模量实验模型, 并在该模型的基础上开展拟南芥根系生长实验。研究发现: 当培养基的杨氏模量在一定范围内0.03~0.12 MPa), 可以根据培养基的杨氏模量大小来预测拟南芥根系的长势。同时, 0.03~0.12 MPa的杨氏模量下, 拟南芥根系生长发育并不是严格地随着培养基杨氏模量的增大而受到抑制。培养基的杨氏模量为0.06 MPa时最适合拟南芥根系生长发育当培养基杨氏模量大于0.06 MPa, 根系生长发育随着杨氏模量的增大而受到抑制, 达到0.11 MPa, 拟南芥根系不能穿透培养基生长。

关键词:杨氏模量; 力学刺激; 拟南芥; 根系发育; 力学模型

收稿:2020-09-01   修定:2020-12-23

资助:国家自然科学基金面上项目(11572063)和重庆大学“重庆市研究生科研创新项目” (CYS18016)

Effects of Young's Modulus of the culture medium on the growth and development of Arabidopsis root system

MENG Run1,2, WANG Bochu1,2,*
1College of Bioengineering, Chongqing University, Chongqing 400044, China; 2Key Laboratory of Biorheological Science and Technology, Ministry of Education, Chongqiang University, Chongqing 400044, China

Corresponding author: WANG Bochu; E-mail: wangbc2000@126.com

Abstract:

The influence of mechanical environment on plant root growth and morphological structure has attracted more and more attention from researchers. However, the current related research focuses on the physiological changes of root system under mechanical stimulation, and there is still a lack of research on quantitative mechanical regulation of root growth and development. At the same time, the mechanical stimulation of the degree of soil compaction will directly affect the growth of the root system, which in turn will affect the growth and development of crops and their yield. Therefore, the establishment of a medium model with controllable mechanical strength to simulate soil conditions have great signifcance for studying the penetration and growth of roots in the soil. In this study, a gradient Young's modulus experimental model was successfully constructed based on Phytagel medium, and the Arabidopsis root system growth experiment was carried out in this model. Results show when the Young's modulus of the culture medium is within a certain range (0.03~0.12 MPa), the root growth of Arabidopsis can be predicted based on the Young's modulus of the culture medium. At the same time, under the Young's modulus of 0.03~0.12 MPa, the growth and development of Arabidopsis root system is not strictly inhibited as the Young's modulus of the medium increases. When the Young's modulus of the medium is 0.06 MPa, it is most suitable for the growth and development of Arabidopsis roots; when the Young's modulus of the medium is greater than 0.06 MPa, the growth and development of the roots will be inhibited as the Young's modulus increases, reaching 0.11 MPa and Arabidopsis roots cannot penetrate the culture medium to grow.

Key words: Young's Modulus; mechanical stimulation; Arabidopsis thaliana; root growth and development; mechanical model

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