《植物生理学报》 2018, 54(11): 1695-1702
摘 要：为研究花生叶片在干旱胁迫后恢复生长的生理和相关基因表达, 本论文采用不同浓度聚乙二醇6000(PEG)模拟干旱条件处理花生幼苗, 复水后观察花生幼苗恢复生长的能力, 并分析在干旱和复水过程中叶片含水量、叶绿素含量、ABA含量以及相关基因表达的变化。结果表明, 10% PEG处理花生幼苗24 h后复水, 不同叶片旱后恢复的能力不同, 嫩叶较老叶恢复能力强; 部分展开叶片在干旱和复水后叶绿素含量均高于正常水平; 而全展开叶片中叶绿素含量在干旱时升高, 复水后降低; 干旱时, 幼叶中的ABA含量显著上升, 复水后降低。而老叶(成熟叶)中的ABA含量持续降低; AhATL1、AhUGT、AhCYP707A1、AhGLK1和AhPORA的表达在干旱时降低, 复水后上调。AhNCED1和AhBG的表达在干旱时升高, 复水后降低。在不同叶片中, 7个基因的变化规律不完全一致, 表明不同叶片响应干旱和复水恢复生长的能力可能与这些基因的表达差异有关。
关键词：花生; 叶片; 干旱胁迫; 复水恢复; 生理特征; 基因
Corresponding author: LI Ling; E-mail: firstname.lastname@example.org
Abstract:To study the physiological and molecular basis of Arachis hypogaea (peanut) leaves during recovery after drought, different concentrations of polyethylene glycol 6000 (PEG) were used to simulate drought condition. Peanut seedlings were treated by PEG, and the recovery ability of peanut seedlings was observed. The relative water content, chlorophyll content, ABA content and the expression of seven genes were analyzed after drought and rewatering. The results showed that 10% PEG was appropriate to treat peanut seedlings for 24 h. The recovery ability of different leaves during recovery from drought was different, showing that young leaves were better than old leaves. The chlorophyll contents of gently unfolded leaves in drought and rewatering process were higher than that of normal condition. However, the chlorophyll content of completely unfolded was increased during drought but decreased after rewatering. ABA content of young leaves was increased significantly during drought, but reduced after rewatering, while the content of ABA in old leaves (mature leaves) was decreased continuously. The expressions of AhATL1, AhUGT, AhCYP707A1, AhGLK1 and AhPORA were decreased during drought and up-regulated after rewatering. In contrast, the expression of AhNCED1 and AhBG were increased during drought but decreased after rewartering. The expression trend of seven genes was inconsistent in different leaves, indicating that the recovery ability of different leaves may be related to the different expressions of these genes.
Key words: Arachis hypogaea (peanut); leaves; drought stress; recovery after rewatering; physiological characteristics; gene
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