《植物生理学报》 2018, 54(11): 1681-1686
摘 要：以金佛山兰子房、柱头等为外植体, 通过丛芽诱导、继代增殖和生根建立离体繁殖体系。结果表明, 丛芽诱导最佳外植体为子房, 最适培养基为MS+2.0 mg•L-1 ZT+0.2 mg•L-1 NAA; 最佳丛芽继代培养基为MS+1.5 mg•L-1 ZT+0.15 mg•L-1 NAA, 1~4代平均繁殖系数为4.76倍, 丛芽长势良好; 最佳生根培养基为1/2MS+0.5 mg•L-1 NAA+0.05 mg•L-1 6-BA, 生根率达100%, 根长为3.95 cm。试管苗移栽到金佛山自然生境, 60 d后的移栽率达60%, 且生长良好。
关键词：金佛山兰; 组织培养; 快速繁殖
Corresponding author: TANG Shao-Hu; E-mail: firstname.lastname@example.org
Abstract:In vitro propagation system was established by using ovary, stigma, etc. of Tangtsinia nanchuanica as explants. The results showed that the optimal explants were ovary, and the optimal medium was MS+2.0 mg•L-1 ZT+0.2 mg•L-1 NAA. The optimal subculture medium was MS+1.5 mg•L-1 ZT+0.15 mg•L-1 NAA. The average reproduction coefficient of 1 to 4 generations was 4.76 times, and the bud grew well. The optimal rooting medium was 1/2MS+0.5 mg•L-1 NAA+0.05 mg•L-1 6-BA with rooting rate of 100% and root length of 3.95 cm. Plantlets in vitro were then transplanted to the natural habitat of Jinfo Mountain, the survival rate was 60% and plants grew well after 60 days.
Key words: Tangtsinia nanchuanica; tissue culture; rapid propagation
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