珍稀濒危植物金佛山兰的组培快繁

童虹宇1, 周小雪1, 李娟1, 邓洪平1, 王茜1, 周启贵1, 汤绍虎1,*, 张军2, 李中娟2, 陈玉菡2
1西南大学三峡库区生态环境教育部重点实验室, 生命科学学院, 重庆400715; 2重庆市药物种植研究所, 重庆408435

通信作者:汤绍虎;E-mail: tangsh@swu.edu.cn

摘 要:

以金佛山兰子房、柱头等为外植体, 通过丛芽诱导、继代增殖和生根建立离体繁殖体系。结果表明, 丛芽诱导最佳外植体为子房, 最适培养基为MS+2.0 mg•L-1 ZT+0.2 mg•L-1 NAA; 最佳丛芽继代培养基为MS+1.5 mg•L-1 ZT+0.15 mg•L-1 NAA, 1~4代平均繁殖系数为4.76倍, 丛芽长势良好; 最佳生根培养基为1/2MS+0.5 mg•L-1 NAA+0.05 mg•L-1 6-BA, 生根率达100%, 根长为3.95 cm。试管苗移栽到金佛山自然生境, 60 d后的移栽率达60%, 且生长良好。

关键词:金佛山兰; 组织培养; 快速繁殖

收稿:2018-08-23   修定:2018-11-05

资助:国家林业和草原局“极小种群野生动植物资源拯救项目” (林规发2016-81号)和国家自然科学基金(31370317)。

Tissue culture and rapid propagation of rare and endangered Tangtsinia nanchuanica

TONG Hong-Yu1, ZHOU Xiao-Xue1, LI Juan1, DENG Hong-Ping1, WANG Qian1,2, ZHOU Qi-Gui1, TANG Shao-Hu1,*, ZHANG Jun2, LI Zhong-Juan2, CHEN Yu-Han2
1Key Laboratory of Eco-Environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China; 2Chongqing Institute of Medicinal Plant Cultivation, Chongqing 408435, China

Corresponding author: TANG Shao-Hu; E-mail: tangsh@swu.edu.cn

Abstract:

In vitro propagation system was established by using ovary, stigma, etc. of Tangtsinia nanchuanica as explants. The results showed that the optimal explants were ovary, and the optimal medium was MS+2.0 mg•L-1 ZT+0.2 mg•L-1 NAA. The optimal subculture medium was MS+1.5 mg•L-1 ZT+0.15 mg•L-1 NAA. The average reproduction coefficient of 1 to 4 generations was 4.76 times, and the bud grew well. The optimal rooting medium was 1/2MS+0.5 mg•L-1 NAA+0.05 mg•L-1 6-BA with rooting rate of 100% and root length of 3.95 cm. Plantlets in vitro were then transplanted to the natural habitat of Jinfo Mountain, the survival rate was 60% and plants grew well after 60 days.

Key words: Tangtsinia nanchuanica; tissue culture; rapid propagation

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