《植物生理学报》 2018, 54(7): 1233-1238
摘 要：T-6b为农杆菌T-DNA上的一个起致瘤作用的基因, 在营养组织中过量表达后能导致突起和肿瘤的形成; 在胚乳中的过表达能提高胚成熟种子的生物量与含油量。本文用油菜中胚特异性表达Napin基因的启动子启动拟南芥T-6b基因的表达。结果显示, 当T-6b在胚表达以后, 种子褶皱, 生物量下降, 种子中多种碳水化合物含量降低。这一结果与T-6b在胚乳中表达的表型结果有所差别。
关键词：T-6b; 胚特异性表达; 种子生物量; 碳水化合物
Corresponding author: LIU Chun-Lin; E-mail: email@example.com
Abstract:As an oncogene located in T-DNA region in Agrobacterium tumefaciens, T-6b can induce enation and tumors with its over-expression in vegetative tissues and increase the biomass and oil contents of mature seeds expressed in endosperm. In this paper, T-6b was fused to the promoter of Napin that could be specially expressed in the embryo of Brassica napus seed. The results showed that with the expression of T-6b in the embryo of Arabidopsis thaliana seed, the seed became wrinkled, and the contents of biomass and carbohydrates were decreased. These results were different from the phenotypic results of T-6b expression in endosperm.
Key words: T-6b; embryo-special expression; seed biomass; carbohydrates
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