植物树脂半薄切片染色方法的改进

李兵, 李登弟, 张杰, 黄耿青, 李学宝*
华中师范大学生命科学学院, 遗传调控与整合生物学湖北省重点实验室, 武汉430079

通信作者:李学宝;E-mail: xbli@mail.ccnu.edu.cn;Tel: 027-67862443

摘 要:

以双子叶植物棉花的幼根、幼茎、叶、胚珠和单子叶植物玉米茎为实验材料, 制作树脂半薄切片, 对样品染色方法 等进行了改进, 将蕃红-固绿染色、苏木精-伊红染色、氯化铁-苏木精染色方法系统应用于植物树脂半薄切片制备中, 获得 了结构完整、染色清晰的棉花各器官和玉米茎半薄切片, 建立了一套适合于植物树脂半薄切片制作的染色方法。

关键词:植物组织; 半薄切片; 染色方法改进

收稿:2011-10-08   修定:2011-10-26

资助:国家自然科学基金(30871317和31070281)。

Modification of Resin Semithin Section Staining Method in Plant Tissues

LI Bing, LI Deng-Di, ZHANG Jie, HUANG Geng-Qing, LI Xue-Bao*
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China

Corresponding author: LI Xue-Bao; E-mail: xbli@mail.ccnu.edu.cn; Tel: 027-67862443

Abstract:

In this study, a new resin semithin section staining method suitable to plant tissue-section was established. Young roots, young stems, leaves and ovules of cotton (Gossypium hirsutum) and stems of maize (Zea mays) were analyzed by the methods of resin semithin section. The improved staining methods (including safranin-fast green staining, hematoxylin-eosin staining, FeCl3-haematoxylin staining) were applied in the preparation of the plant semithin section. Our experimental results indicated that clear-staining and integral-structural semithin section of cotton and maize tissues were obtained by using modified methods of resin semithin section.

Key words: plant tissue; semithin section; improvement of staining methods

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