《植物生理学报》 2017, 53(12): 2137-2146
摘 要：兰花的试管苗在移栽前一般需要炼苗以提高其移栽成活率。本文以魔帝兜兰(Paphiopedilum Maudiae)的试管苗、温室中炼苗10 d后的试管苗及移栽不同时间(15、30、45 d)的幼苗为材料, 研究炼苗对其移栽成活率的影响以及炼苗和移栽过程中叶片气孔及光合特性的变化。结果表明, 炼苗对叶片的气孔数量、密度和开放率有较大影响, 但对移栽成活率无显著影响。试管苗在移栽30 d时叶片气孔数量增加, 气孔密度增大, 叶片生长势强; 移栽45 d时气孔大小、密度以及气孔指数均减小, 气孔首次出现午休现象, 即在中午14:00气孔关闭, 然后在17:00后又开放, 气孔开放与光合速率日变化的趋势一致。由此推测, 魔帝兜兰试管苗可不经炼苗过程直接进行移栽, 约45 d后能适应温室环境。
关键词：魔帝兜兰; 气孔; 光合作用
Corresponding author: ZENG Song-Jun; E-mail: firstname.lastname@example.org
Abstract:It is usually necessary to harden in vitro plantlets in the greenhouse before transplanting to improve their survival. In this paper, the level of survival after transplanting in vitro-derived Paphiopedilum Maudiae plantlets, as well as leaf stomatal variation and photosynthetic characteristics, were studied. Plantlets were hardened in culture flasks in a greenhouse for 10 d, then transplanted to a greenhouse for 15, 30 or 45 d. The number, density and opening rate of stomata were observed and measured. Results showed significant differences in all three parameters between in vitro plantlets and plantlets hardened in the culture flask in a greenhouse for 10 d, but the level of post-transplant survival was not significantly different. There was an increase in the number of stomata and stomatal density of plantlets 30 d after transplanting, while the number of stomata, stomatal density and opening rate of leaves decreased and many stomata closed at midday in plantlets 45 d after transplanting. These results suggest that in vitro-derived Paphiopedilum Maudiae plantlets need not be hardened when transplanted to a greenhouse, and that in vitro plantlets could adapt to the greenhouse environment after 45 d of transplanting.
Key words: Paphiopedilum Maudiae; stomata; photosynthesis
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