丛枝菌根网对喀斯特适生植物氮、磷化学计量特征的影响

杨应1, 蒋长洪2, 何跃军1,*, 欧静1, 王鹏鹏1, 司建朋1, 何敏红1, 林艳1
1贵州大学林学院, 贵阳550025; 2贵阳市城市绿化管理处, 贵阳550001

通信作者:何跃军;E-mail: hyj1358@163.com

摘 要:

为探究丛枝菌根真菌根外菌丝连接所形成的地下菌丝网络对不同喀斯特适生植物矿质元素化学计量特征的影响, 本试验以3种喀斯特适生植物香樟(Cinnamomum camphora)、构树(Broussonetia papyrifera)和鬼针草(Bidens pilosa)为研究对象, 种植于由7个隔室组成的试验装置中, 将试验单元分为接种AM香樟隔室和未接种AM植物隔室, 采用20和0.45 μm尼龙网对未接种AM植物隔室进行处理, 苗木培养4个月后收获测定植物根、茎、叶及土壤N、P含量及苗木生物量, 计算N、P摄取量。结果表明: 丛枝菌根网显著提高了香樟、构树和鬼针草叶中N和P含量, 表现为鬼针草>构树>香樟, 并显著降低了3种植物叶片氮磷比。丛枝菌根网显著提高了香樟根、茎、叶的N、P摄取量, 但对构树、鬼针草各部位N摄取量影响不显著, 根、茎、叶的N、P摄取量分配比例存在差异。在未接种AM植物隔室中, 丛枝菌根菌丝网显著提高了20 μm香樟隔室中土壤全P含量, 显著降低了香樟和鬼针草20 μm隔室土壤氮磷比。植物与土壤N、P相关性分析结果表明, 植物N摄取量与植物茎、叶N含量和茎P含量显著相关, 植物根N、P含量与土壤全N含量相关性显著, 土壤全P分别与植物叶N及茎、叶P含量间显著相关。本研究表明丛枝菌根菌丝网不同程度地影响了3种植物对N、P养分的吸收, 显著降低了各植株叶片氮磷比, 一定程度缓解了3种植物营养生长限制。

关键词:丛枝菌根; 菌丝网; N、P含量; 化学计量; 喀斯特

收稿:2017-04-13   修定:2017-11-17

资助:国家自然基金(31360106、31660156、31560223)、贵州省农业攻关项目(黔科合NY[2014]3029号)、贵州省优秀青年科技人才专项基金[黔科合人字2013(10)]、贵州省科技计划项目(黔科合[2016]支撑2805)、贵州省生态学重点学科建设项目(黔学位合字ZDXK[2016]7号)和贵州大学研究生创新基金(研农2017011)。

Effects of arbuscular mycorrhizal networks on the N and P contents and stoichiometry of three plants species from Karst area

YANG Ying1, JIANG Chang-Hong2, HE Yue-Jun1,*, OU Jing1, WANG Peng-Peng1, SI Jian-Peng1, HE Ming-Hong1, LIN Yan1
1College of Forestry, Guizhou University, Guiyang 550025, China; 2The Management Office of the Urban Greening of Guiyang, Guiyang 550001, China

Corresponding author: HE Yue-Jun; E-mail: hyj1358@163.com

Abstract:

To investigate how arbuscular mycorrhizal networks affect mineral element contents and stoichiometric characteristics of different adaptative plants in karst areas, three Karst species, Cinnamomum camphora, Broussonetia papyrifera and Bidens pilosa were selected in this study. They were planted in an experimental device composed of seven compartments. The central one was AM fungus inoculated C. camphora treated with 20 μm nylon mesh, the other six compartments were non-inoculated species (treated with 20 μm and 0.45 μm nylon mesh, respectively). After 4 months’ cultivation, N and P concentrations of plant roots, stem, leaves and soil and also the biomass of roots and stems and leaves were measured. The results showed that arbuscular mycorrhizal network increased N and P contents in leaves of the three plants significantly, which ranked as Bi>Br>Ci Arbuscular mycorrhizal networks decreased the N/P ratio in leaves of the three plants significantly. In addition, arbuscular mycorrhizal networks significantly increased the N and P acquisition in roots, stems and leaves of Ci, but no significant differences were found in Br and Bi. Arbuscular mycorrhizal networks significantly increased the soil total P content of non-inoculated Ci compartment, and significantly decreased the soil N/P ratio of Ci and Bi in non-inoculated compartments. N acquisition of plant individuals was significantly correlated to N content in leaves and stems and P content in stems. Moreover, significant correlations were found among soil total N and N, P contents in roots, among soil total P, N, P contents in leaves and P contents in stems. This study indicated that arbuscular mycorrhizal networks alleviated the nutrition restriction of plant growth through their different impacts on N and P uptake and N/P ratio of leaves for the three plants.

Key words: arbuscular mycorrhizae; mycorrhizal networks; contents of N and P; stoichiometry; Karst

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