干旱胁迫训练对花生生长及胁迫相关基因表达的影响

陈容钦, 舒文, 葛奎, 胡博*, 李玲
华南师范大学生命科学学院, 广东省植物发育生物工程重点实验室, 广州510631

通信作者:胡;E-mail: hubo0610@126.com

摘 要:

本文分析了重复脱水训练对四叶期花生(Arachis hypogaea)生理表型和相关基因表达的影响, 发现第二次干旱胁迫(D2)过程中花生植株叶片萎蔫程度及茎弯曲程度较第一次干旱胁迫(D1)低, 第二次复水(R2)中花生茎弯曲程度恢复比第一次复水(R1)提前; D2、R2的相对含水量分别较D1、R1低; D1的完全开放气孔比例相对D2的变化幅度大, R1的完全闭合气孔比例也比R2低; D2中AhNCED1AhDHN1AhATL1基因表达量明显比D1的表达量高。结果表明, 花生具有干旱胁迫记忆, 体内干旱相关基因经干旱重复训练表达量升高, 进而增强花生旱后恢复能力。

关键词:干旱重复训练; 花生; 生长; 抗旱基因表达

收稿:2017-08-08   修定:2017-09-21

资助:国家自然科学基金(31471422)和华南师范大学“挑战杯”金种子培育项目(16SKKB02)。

Effect on growth and expressions of stress-related genes in peanut under drought stress training

CHEN Rong-Qin, SHU Wen, GE Kui, HU Bo*, LI Ling
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China

Corresponding author: HU Bo; E-mail: hubo0610@126.com

Abstract:

The expression changes of drought response genes and phenotypic properties of physiology in peanuts (Arachis hypogaea) under repeated dehydration training were analyzed in this paper. The leaf wilting and curve degree of stem in peanut in D2 (repeated dehydration) treatment were lighter than that in D1 (dehydration of the first time) treatment. The curve degree of stem in R2 recovered earlier than that in R1. The percentages of leaf water content in D2 and R2 were respectively lower than those in D1 and R1. The variation range of completely open stoma ratio in D1 was higher than that in D2, and the completely closed stoma ratios in R1 were lower than that in R2. The expression levels of stress-related genes, AhNCED1, AhDHN1 and AhATL1, in D2 were higher than those in D1 plants. These results indicate that peanut plants possess drought stress memory. The expression levels of drought stress-related genes were increased under drought stress training, further enhancing drought tolerance and recovery ability after the release of drought.

Key words: drought stress training; peanut; growth; stress-related gene expression

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