烟草NTHK1基因提高杨树根系的耐盐性

王鹏1, 杨迎霞2, 宋建2, 张越2, 陈受宜3, 王景安1,*, 刘仲齐2,*
1天津师范大学生命科学学院, 天津300387; 2天津市农业生物技术研究中心, 天津300384; 3中国科学院遗传与发育生物学研究所, 北京100101

通信作者:王景安;E-mail: zqliu733@yahoo.com.cn, jinganwang899@;Tel: 022-23733857, 022-27950688

摘 要:

以受体杨树‘107号’和转入NTHK1 (Nicotiana tabacum histidine kinase-1)基因的‘18-1’及‘18-4’的水培苗为材料, 不同浓 度的NaCl胁迫12 d后, 发现‘18-1’及‘18-4’的根长和根重显著大于‘107号’。以离体根段为材料, 在400 mmol•L-1NaCl溶液中 胁迫60 min后, ‘107号’的K+外渗量比‘18-1’和‘18-4’分别高49.34%和19.68%; 在400 mmol•L-1 KCl或NaCl胁迫30 min, ‘107号’ 的电解质外渗率(REL)显著大于‘18-1’和‘18-4’; 等渗的30% PEG对离体根段的REL影响很小。在400 mmol•L-1 NaCl溶液中 添加200~400 mmol•L-1的KNO 或KCl能显著增加离体根段的REL, 并使不同基因型的REL差异更大。这表明, 测定离体根 段的K+外渗量和REL可以快速鉴定不同基因型杨树的耐盐潜力。

关键词:杨树; 根段; K+外渗量; 电解质外渗率; 耐盐性

收稿:2011-07-07   修定:2011-11-10

资助:国家重点基础研究发展计划(2006CB100102)、“十一五” 国家科技支撑计划(2009BADA3B01)和国际科技合作项目 (2011DFA30990)

NTHK1 of Tobacco Improves Salt Tolerance of Roots in Poplars

WANG Peng1, YANG Ying-Xia2, SONG Jian2, ZHANG Yue2, CHEN Shou-Yi3, WANG Jing-An1,*, LIU Zhong-Qi2,*
1Department of Biology, Tianjin Normal University, Tianjin 300387, China; 2Tianjin Research Center of Agricultural Bio-technology, Tianjin 300384, China; 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

Corresponding author: WANG Jing-An; E-mail: zqliu733@yahoo.com.cn, jinganwang899@; Tel: 022-23733857, 022-27950688

Abstract:

Effects of different concentrations of NaCl on salt tolerance of roots in poplar ‘107’ (Populus×euramericana ‘Neva’) and transgenic poplar ‘18-1’ and ‘18-4’ with NTHK1 (Nicotiana tabacum histidine kinase-1) were investigated. The results showed that dry weight and length of roots in ‘18-1’ and ‘18-4’ were significantly higher than that of ‘107’. Under the stress of 400 mmol·L-1 NaCl for 60 min, the K+ leakage amount of ‘107’ were 49.34% and 19.68% higher than those of ‘18-1’ and ‘18-4’ respectively. And the relative electrolyte leakage (REL) of ‘107’ was also significantly higher than those of ‘18-1’ and ‘18-4’ after treatments of 400 mmol·L-1 KCl or NaCl for 30 min. But isotonic 30% PEG had little effect on the REL of root segment in vitro. The NaCl solution added with 400 mmol·L-1 KCl or KNO3 significantly increased the REL of root segment in vitro, and made a greater difference between the different genotypes poplar. The results showed that the determination of K+ leakage volume and REL of root segments in vitro could quickly identify potential salt tolerance of different genotypes of poplars.

Key words: poplar; root system; K+ leakage; electrolyte leakage; salt tolerance

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