小桐子JcNF-YB3JcNF-YB10基因的克隆与表达分析

黄尧瑶1, 邓明华3, 施卫省2, 龚明4, 陈浩维1, 文锦芬2,*
昆明理工大学1现代农业工程学院, 2建筑与城市规划学院, 昆明650500; 3云南农业大学园林园艺学院, 昆明650201; 4云南师范大学生命科学院, 昆明650500

通信作者:文锦芬;E-mail: wenjf888@163.com

摘 要:

以小桐子(Jatropha curcas) cDNA为模版, 克隆了JcNF-YB3JcNF-YB10基因的CDS序列。序列分析表明该两个基因分别包含549和528 bp完全阅读框(ORF), 编码182和175个氨基酸。Blast搜索结果及进化分析结果表明, JcNF-YB3和JcNFYB10都与油桐(Vernicia fordii) NF-YB家族基因序列一致性最高且亲缘关系最近。定量分析表明, JcNF-YB3JcNF-YB10在小桐子根、茎、叶、花、果皮和种子中都有表达, JcNF-YB3在根中表达量最高, JcNF-YB10在种子中表达量最高。两个基因在种子发育期的表达量变化趋势一致, 但是JcNF-YB10在种子整个生长发育期的表达量都要高于JcNF-YB3JcNFYB3JcNF-YB10都在NaCl、PEG、4°C低温和ABA处理后表达量不同程度上调, 推测这两个基因在小桐子中参与抗逆反应机制和植物激素信号传导。

关键词:小桐子; JcNF-YB3; JcNF-YB10; 克隆; 生物信息学分析; 表达分析

收稿:2016-12-05   修定:2017-03-15

资助:国家自然科学基金(31460355)。

Cloning and expression analysis of JcNF-YB3 and JcNF-YB10 from Jatropha curcas

HUANG Yao-Yao1, DENG Ming-Hua3, SHI Wei-Sheng2, GONG Ming4, CHEN Hao-Wei1, WEN Jin-Fen2,*
1Faculty of Modern Agricultural Engineering, 2Faculty of Architecture and City Planning, Kunming University of Science and Technology, Kunming 650500, China; 3College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China; 4School of Life Sciences, Yunnan Normal University, Kunming 650500, China

Corresponding author: WEN Jin-Fen; E-mail: wenjf888@163.com

Abstract:

Two genes of NF-YB family (JcNF-YB3 and JcNF-YB10) were isolated from Jatropha curcas. The opening reading frame (ORF) of JcNF-YB3 was 549 bp, encoded 182 amino acids. The opening reading frame (ORF) of JcNF-YB10 was 528 bp, encoded 175 amino acids. The results of homology analysis showed that both JcNF-YB3 and JcNF-YB10 had the highest identity and the closest relationship with NF-YB family of Vernicia fordii. JcNF-YB3 and JcNF-YB10 expression significantly in different organisms, JcNF-YB3 had the highest transcript profile in root, JcNF-YB10 had the highest transcript profile in seed, the expression level of JcNFYB10 was a lot higher than that of JcNF-YB3 during seed growth periods. The expression level of JcNF-YB3 and JcNF-YB10 were both induced by NaCl, PEG, low temperature and abscisic acid (ABA) and those treatments upregulated JcNF-YB3 and JcNF-YB10 gene expression. The results suggested that JcNF-YB3 and JcNFYB10 both involved in response abiotic stress and signal transduction pathway of plant hormones in J. curcas.

Key words: Jatropha curcas; JcNF-YB3; JcNF-YB10; clone; bioinformatics analysis; expression analysis

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