马铃薯块茎创伤木栓化过程中形态生理指标及内源激素的变化

杨芳, 李俊, 郭华春*
云南农业大学农学与生物技术学院, 昆明650201

通信作者:郭华春;E-mail: ynghc@126.com

摘 要:

本试验以马铃薯(Solanlum tuberosum)完整块茎为对照, 马铃薯块茎纵切为创伤处理, 主要研究马铃薯块茎创伤愈合过程中块茎内源激素、木质素合成关键酶(苯丙氨酸解氨酶、过氧化物酶)及创伤周皮形态解剖结构的变化。实验结果表明马铃薯块茎受创后苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)酶活明显增加, 内源激素脱落酸(ABA)和玉米素核苷(ZR)含量明显增加, 而赤霉酸(GA)含量降低。块茎创伤处理后创面在第4天形成木栓形成层, 在第10天进入创伤周皮稳定增长的时期。同时, 在处理块茎的未创伤处, 其周皮厚度和层数均优于对照。实验结果表明马铃薯块茎创伤后由于ABA、ZR和GA等内源激素协同调控及POD、PAL酶活增加, 促进了创伤周皮的形成, 使受创的马铃薯块茎完成创伤木栓化。

关键词:马铃薯; 创伤木栓化; 内源激素; 苯丙氨酸解氨酶; 创伤周皮

收稿:2017-02-24   修定:2017-05-15

资助:国家自然科学基金(31660355)和国家现代农业产业技术体系(CARS-10-P21)。

Changes of morphological and physiological indices and endogenous hormones during potato tuber wound-healing suberization

YANG Fang, LI Jun, GUO Hua-Chun*
College of Agronomy and Biotechnology, Yunnan Agriculture University, Kunming 650201, China

Corresponding author: GUO Hua-Chun; E-mail: ynghc@126.com

Abstract:

In this study, we investigated the changes of endogenous hormones, the activities of relevant enzymes of lignin synthesis including phenylalanine ammonia-lyase (PAL) and peroxidase (POD), and the anatomical structure of the wound periderm during potato (Solanlum tuberosum) wound-healing suberization. After wound treatment PAL and POD activities were significantly increased and the contents of abscisic acid (ABA) and zeatin riboside (ZR) were higher than untreated control tubers, but the content of gibberellic acid (GA) was lower than untreated ones. During the day 3–4, phellogen formed on the hurt side of the tubers. The cell layers of peridem was 6 and the periderm increased steadily in day 10. Meanwhile the thickness and layers of periderm in the unhurt areas of treated tuber were higher than the control. The experimental results show that the regulation of some endogenous hormones and the increase of PAL and POD activities promoted the wound-healing suberization.

Key words: Solanlum tuberosum; wound-healing suberization; endogenous hormone; phenylalanine ammonialyase; wound-healing periderm

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