过表达GmATG8c基因提高大豆的低氮耐受性和产量

徐伟, 刘生, 游翔, 梅圆圆, 王丹*, 王宁宁*
南开大学生命科学学院, 天津300071

通信作者:王;E-mail: wangdan629@nankai.edu.cn; wangnn@nankai.edu.cn

摘 要:

大豆是一种高需氮素作物, 其籽粒发育所需氮素在很大程度上来源于“源”器官中氮素的再动员, 而细胞自噬是植物进行氮素再动员的主要形式之一。为了研究大豆细胞自噬关键基因GmATG8c的功能, 本文利用35S启动子驱动GmATG8c基因在大豆中过表达。通过农杆菌介导的大豆子叶节转化法获得了3个独立的单拷贝35S:GmATG8c转基因大豆株系, 半定量RT-PCR结果显示, 所有转基因株系中目的基因均得到了高效表达。进一步对转基因大豆株系进行的低氮耐受性和产量相关性状分析结果表明, 在低氮培养条件下, 35S:GmATG8c转基因大豆的生长速率加快, 二级分枝数和单株粒数增多, 百粒重增加, 单株产量显著提高。说明GmATG8c是一个具有提高NUE (nitrogen use efficiency)育种潜力的基因。

关键词:大豆; 细胞自噬; GmATG8c; 低氮耐受性; 产量

收稿:2016-12-21   修定:2016-12-28

资助:国家转基因生物新品种培育科技重大专项(2016ZX0800-4005-004和2014ZX0800930B-002)和国家自然科学基金(31370285)。

Overexpression of GmATG8c gene enhances tolerance to low nitrogen and increases yield in soybean

XU Wei, LIU Sheng, YOU Xiang, MEI Yuan-Yuan, WANG Dan*, WANG Ning-Ning*
College of Life Sciences, Nankai University, Tianjin 300071, China

Corresponding author: WANG Dan; E-mail: wangdan629@nankai.edu.cn; wangnn@nankai.edu.cn

Abstract:

Soybean is in great demand for nitrogen in order to sustain its growth and development. Nitrogen needed for its grain development is largely derived from the remobilization in vegetative organs, of which autophagy is one of the main forms in plants. To functionally characterize the key autophagy gene in soybean, GmATG8c, we used 35S promoter to drive the expression of this gene and obtained three independent transgenic lines of single copy via Agrobacterium-mediated transformation method. RT-PCR analysis showed that exogenous GmATG8c gene was overexpressed in all transgenic lines. Further phenotypic analysis demonstrated that 35S:GmATG8c transgenic lines got accelerated growth rate, more secondary branches and seed number per plant, increased hundred-grain weight and significantly increased yield per plant under low nitrogen conditions. All results in the current study suggest that GmATG8c is with NUE potential, which can be used to improve the efficiency of nitrogen utilization in soybean.

Key words: soybean; autophagy; GmATG8c; low nitrogen tolerance; yield

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