《植物生理学报》 2016, 52(10): 1555-1564
摘 要：为揭示白杨派无性系对盐渍环境的适应能力, 筛选速生耐盐碱优良无性系, 本文采用盆栽试验方法, 对8个白杨派无性系在不同浓度(0、30、60、90 mmol•L-1) NaHCO3胁迫下的生长及生理代谢指标的变化进行了研究。结果表明: 8个无性系的地上部分和根系生长均受到明显抑制, 无性系26和89表现出较强的速生性; 叶绿素含量均呈下降趋势, 其中下降最小的为无性系26和89; 随着NaHCO3处理浓度的增加, 8个无性系叶片脯氨酸含量持续增加, 其中无性系26和89显著高于相同胁迫条件下其他无性系; NaHCO3胁迫导致叶片细胞膜相对透性以及丙二醛(MDA)含量提高, 但相同胁迫条件下无性系26、LM50、89细胞膜相对透性以及MDA含量均显著低于其他无性系。超氧化物岐化酶(SOD)活性呈先增大后减小的变化趋势, 其中无性系89和26的酶活性始终高于其他无性系; 在90 mmol•L-1 NaHCO3处理下, 苗高增长量、地径增长量与各生理指标之间的相关性均达显著或极显著相关, 表明利用生长指标和生理指标对无性系耐盐性进行综合评价, 更能反映NaHCO3胁迫后的响应。利用主成分分析对8个无性系生长及生理代谢等参数进行综合评价, 各无性系耐盐碱性由强到弱依次为26、89、LM50、150、Z3、27、1316、Y3。因此, 初步认为无性系26和89具有显著耐盐碱性和速生性, 可作为盐渍立地造林或抗逆育种的重要试验材料应用。
关键词：白杨派; NaHCO3胁迫; 生长; 生理指标
Corresponding author: LI Shan-Wen; E-mail: firstname.lastname@example.org
Abstract:In order to explore the responses of white poplar clones to NaHCO3 stress and select superior clones with characteristics of fast-growth and strong saline-alkaline resistance, the growth and physiological characteristics of eight white poplar clones grown in pots under 0, 30, 60 and 90 mmol•L-1 NaHCO3 stresses were investigated. The results showed that the growth of above-ground and root were inhibited apparently under NaHCO3 stress. The two clones 26 and 89 showed the fast-growth. The chlorophyll content decreased obviously in all clones, and the chlorophyll content of clone 26 and 89 showed the smallest decline under NaHCO3 stress. The content of the proline in eight clones showed the trend of increasing gradually with the increase of NaHCO3 concentration, and that of clone 26 and 89 was significantly higher than the other clones. The cell membrane permeability and MDA content had a steady increase with the increase of NaHCO3 concentration, and that of clone 26, LM50 and 89 was significantly lower than others under the same stress. SOD activity firstly increased and then decreased, and that of clone 26 and 89 was always higher than the other clones. Under 90 mmol•L-1 NaHCO3 stress, the height increment and the basal diameter increment had a significantly correlation with all the physiological indexes. It indicated that using the growth and physiological indexes to evaluate the salt-resistance of eight clones could therefore reflect the response of NaHCO3 stress. Through principal component analysis, the sequence of salt resistance in eight clones was as follows: 26>89>LM50>150>Z3>27>1316>Y3. Therefore, it was preliminarily considered that clone 26 and 89 had significant saline-alkaline resistance and fast-growing, which could be used as the important testing materials for afforestation in saline area and breeding resource in resistance research.
Key words: Leuce; NaHCO3 stress; growth; physiological index
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