《植物生理学报》 2016, 52(9): 1443-1448

海伦兜兰的无菌播种与快速繁殖

胡琦敏^1,2, 李勇毅³, 黄云峰^1,2,*, 胡仁传¹, 吴坤林⁴

¹广西壮族自治区中医药研究院, 南宁530022; ²广西中药质量标准研究重点实验室, 南宁530022; ³自然科学博物馆, 台湾台中404; ⁴中国科学院华南植物园华南农业植物分子分析与遗传改良重点实验室, 广州510650

通信作者：黄云峰；E-mail: huangyunfeng2000@126.com

摘　要：

本文研究了海伦兜兰(Paphiopedilum helenae)的无菌播种与快速繁殖, 结果表明: 提高海伦兜兰种子萌发率较好的预处理方法是用0.1 mol•L^-1 KOH处理10min; 最适合海伦兜兰种子萌发的培养基为1/4MS+0.1% (m/V)活性炭+60 g•L^-1香蕉(Musa nana), 种子萌发率达69.4%; 最适合丛生芽诱导的培养基为1/2MS+0.5 mg•L^-1 6-苄氨基嘌呤(6-BA)+2.0 mg•L^-1萘乙酸(NAA)+0.1%活性炭+60 g•L^-1香蕉; 最适合生根壮苗的培养基为MS+60 g•L^-1马铃薯(Solanum tuberosum)或MS+30 g•L^-1香蕉。

关键词：海伦兜兰; 种子; 无菌播种

收稿：2016-06-20   修定：2016-08-05

资助：国家重点研发计划项目(2016YFC0503104)、广西自然科学基金(2012GXNSFBA053079)和南宁市青秀区科学研究与技术开发计划课题(2013S13)。

Aseptic seeding and rapid propagation of Paphiopedilum helenae

HU Qi-Min^1,2, LI Yong-Yi³, HUANG Yun-Feng^1,2,*, HU Ren-Chuan¹, WU Kun-Lin⁴

¹Guangxi Institute of Chinese Medicine & Pharmaceutical Science, Nanning 530022, China; ²Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Nanning 530022, China; ³Museum of Natural Science, Taizhong, Taiwan 404, China; ⁴Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China

Corresponding author: HUANG Yun-Feng; E-mail: huangyunfeng2000@126.com

Abstract:

In this study, we investigated aseptic seeding and rapid propagation of Paphiopedilum helenae. The results show that the best method for pretreatment of P. helenae seeds was to soak them into 0.1 mol•L^-1 KOH for 10 min; the best medium for seeds germination was 1/4MS+0.1% (m/V) activated carbon (AC)+ 60 g•L^-1 banana (Musa nana), and the germinating rate was up to 69.4%; the best medium for multiple shoots induction was 1/2MS+0.5 mg•L^-1 6-benzylaminopurine (6-BA)+2.0 mg•L^-1 1-naphthaleneacetic acid (NAA)+0.1% AC+60 g•L^-1 banana; the best medium for rooting and growth of plantlet was MS+60g•L^-1 potato (Solanum tuberosum) or MS+30 g•L^-1 banana.

Key words: Paphiopedilum helenae; seed; aseptic seeding