《植物生理学报》 2016, 52(9): 1379-1388

NaCl胁迫对中山杉幼苗生长及离子吸收、运输和分配的影响

於朝广^1,*, 李颖², 谢寅峰², 殷云龙¹

¹江苏省中国科学院植物研究所, 南京210014; ²南京林业大学生物与环境学院, 南京210037

通信作者：於朝广；E-mail: cg168@sina.com

摘　要：

采用温室盆栽控制试验, 研究0、50、100和150 mmol•L^-1 NaCl胁迫对‘中山杉302’和‘中山杉406’一年生扦插苗的生长和矿质离子吸收、运输和分配的影响。结果表明: (1) 50 mmol•L^-1 NaCl胁迫下‘中山杉302’苗木相对生长量显著高于对照, 而‘中山杉406’与对照无显著差异; 150 mmol•L^-1 NaCl胁迫下, ‘中山杉406’苗木相对生长量和存活率均高于‘中山杉302’, 说明‘中山杉302’对低盐胁迫表现出较强的盐适应力, 而‘中山杉406’对高盐胁迫抗性较强; (2) 50 mmol•L^-1 NaCl胁迫下‘中山杉302’茎、叶能维持相对较低的Na⁺水平, 同时由根向地上部分选择性运输K⁺、Ca²⁺、Mg²⁺的能力较强; 150 mmol•L^-1 NaCl胁迫下, ‘中山杉406’则比‘中山杉302’具有更强的选择性吸收和运输K⁺、Ca²⁺、Mg²⁺的能力, 以保持植物体内Na⁺与K⁺、Ca²⁺、Mg²⁺的离子之间平衡, 2个不同无性系在不同盐浓度胁迫下的适应性差异与其对矿质元素吸收及平衡特性有关。

关键词：中山杉; NaCl胁迫; 生长; 离子平衡

收稿：2016-07-06   修定：2016-08-01

资助：江苏省农业科技自主创新资金项目[CX(13)2046]和江苏省创新能力建设计划(BM2015019)。

Effects of NaCl stress on growth and absorption, transportation and distribution of ions in zhongshanshan seedlings

YU Chao-Guang^1,*, LI Ying², XIE Yin-Feng², YIN Yun-Long¹

¹Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China; ²College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, China

Corresponding author: YU Chao-Guang; E-mail: cg168@sina.com

Abstract:

The effects of different concentrations (0, 50, 100, and 150 mmol•L^-1) of NaCl stress on growth, ions (Na⁺, K⁺, Ca²⁺, Mg²⁺) absorption, transportation and distribution in roots, stems and leaves of Taxodium ‘zhongshanshan 302’ and ‘zhongshanshan 406’ in a pot experiment were studied. The results showed that: (1) The relative growth of biomass of ‘zhongshanshan 302’ was significantly higher than controls under 50 mmol•L^-1 NaCl stress, however, there was no significant difference between treatment and the control for ‘zhongshanshan 406’. Under 150 mmol•L^-1 NaCl stress, the seedling relative growth rates and survival rates of ‘zhongshanshan 406’ were higher than that of ‘zhongshanshan 302’. It means ‘zhongshanshan 302’ is more adapted to low salt stress, but ‘zhongshanshan 406’ is more resistance to high salt stress. (2) ‘zhongshanshan 302’ can maintain relative low Na⁺ level in stems and leaves under 50 mmol•L^-1 NaCl stress, and it had a relative strong ability to selectivily transport K⁺, Ca²⁺, and Mg²⁺ from the root to the aboveground. Under 150 mmol•L^-1 NaCl stress, ‘zhongshanshan 406’ had more strong ability to selectively absorb and transport K⁺, Ca²⁺, and Mg²⁺, and to keep the balance between Na⁺ and these ions. The adaptive differences between two clones to the stress of different NaCl concentrations are related to the characteristics of ion absorption and homeostasis.

Key words: Taxodium zhongshanshan; NaCl stress; growth; ion homeostasis