《植物生理学报》 2016, 52(6): 933-940

钙调素参与细菌信号3-羰基辛酰基高丝氨酸内酯对拟南芥主根生长的调控

赵芊^1,2, 张超¹, 刘方^1,2, 贾振华^1,2, 宋水山^{1, 2,*}

¹河北省科学院生物研究所, 石家庄 050051; ²河北省农作物主要病害微生物控制工程技术研究中心, 石家庄050051

通信作者：宋水山；E-mail: shuishans620@163.com

摘　要：

3-羰基辛酰基高丝氨酸内酯(3OC8-HSL)是革兰氏阴性细菌产生的群体感应信号分子, 它能够被植物感知进而促进植物根的生长。本文通过药理学、遗传学和分子生物学技术分析了钙调素(CaM)在3OC8-HSL调控植物根生长中的作用, 发现CaM抑制剂W7和TFP能够阻断3OC8-HSL对拟南芥根长的促进效应, 3OC8-HSL处理能够诱导拟南芥CaM蛋白积累和CaM 7个亚型基因的上调表达, CaM各亚型基因的突变体对3OC8-HSL的促根长效应不敏感, 表明CaM参与3OC8-HSL对拟南芥主根生长的调控。

关键词：钙调素; N-酰基高丝氨酸内酯; 主根伸长

收稿：2016-01-13   修定：2016-05-20

资助：国家重点基础研究发展计划项目(2015CB150600)、国家自然科学基金(31270880)和河北省科学院高层次人才项目(20150503 LR62-10)。致谢 河北师范大学崔素娟教授惠赠cam2、cam3和cam4突变体种子, 孙大业教授惠赠CaM多克隆抗体。

Involvement of calmodulin in regulation of primary root elongation by N-3-oxo-octanoyl homoserine lactone in Arabidopsis thaliana

ZHAO Qian^1,2, ZHANG Chao¹, LIU Fang^1,2, JIA Zhen-Hua^1,2, SONG Shui-Shan^1,2,*

¹Biology Institute, Hebei Academy of Sciences, Shijiazhuang 050051, China; ²Hebei Engineering and Technology Center of Microbiological Control on Main Crop Disease, Shijiazhuang 050051, China

Corresponding author: SONG Shui-Shan; E-mail: shuishans620@163.com

Abstract:

N-3-oxo-octanoyl homoserine lactone (3OC8-HSL) is a primary QS signal among Gram-negative bacteria. Recent evidence shows that 3OC8-HSL is able to be perceived by plants and to promote plant root growth. In this study, the function of calmodulin (CaM) in 3OC8-HSL-induced root elongation was analyzed by pharmacological, genetical and molecular approaches. We found that the stimulatory effect of 3OC8-HSL on primary root elongation of Arabidopsis was abolished by the CaM antagonists N-(6-aminohexyl)- 5-chloro-1-naphthalene sulfonamide (W7) and trifluoperazine (TFP). Western-blot analysis revealed that the concentration of CaM protein in Arabidopsis roots increased after treatment with 10 μmol•L^-1 3OC8-HSL. Results from quantitative RT-PCR demonstrated that the transcription of all seven CaM genes in Arabidopsis genome was up-regulated in the plants treated with 3OC8-HSL. The loss-of-function mutants of each AtCaM gene (AtCaM1–AtCaM7) were insensitive to 3OC8-HSL-stimulation of primary root elongation. Taken together, our results suggested that CaM was involved in the bacterial 3OC8-HSL signaling in plant cells.

Key words: calmodulin; N-acyl-homoserine lactone; primary root elongation