《植物生理学报》 2016, 52(6): 926-932

拟南芥离体器官再生过程中CUC1的DNA甲基化修饰状态分析

宋玉光, 马宗琪, 邱念伟, 董蔚^*

曲阜师范大学生命科学学院, 山东曲阜273165

通信作者：董；E-mail: Dongwei0207@163.com

摘　要：

在植物的生长发育过程中, DNA甲基化修饰是影响基因表达的重要调控因子。CUP-SHAPED COTYLEDON 1 (CUC1)是拟南芥离体器官再生的关键基因。本研究采用实时定量PCR (qRT-PCR)和亚硫酸氢盐测序PCR (BSP)技术检测了拟南芥离体器官再生过程中CUC1的表达与其DNA甲基化修饰的动态调控关系, 发现CUC1的表达与其启动子区和3′端的DNA甲基化修饰水平呈明显负相关性。本研究为深入了解CUC1参与拟南芥离体器官再生的可能调控机制提供参考。

关键词：拟南芥; DNA甲基化; CUC1; 离体器官再生

收稿：2016-03-03   修定：2016-05-15

资助：国家自然科学基金青年基金(31300220和31501328)、山东省自然科学基金(2015ZRB01APV和ZR2014CP013)、中国博士后基金面上项目(2015M572000和2014M550366)和曲阜师范大学精品实验及实验室开放基金(SK201501和Jp2015018)。

DNA methylation modification status of CUC1 during in vitro organogenesis in Arabidopsis thaliana

SONG Yu-Guang, MA Zong-Qi, QIU Nian-Wei, DONG Wei^*

College of Life Sciences, Qufu Normal University, Qufu, Shandong 273165, China

Corresponding author: DONG Wei; E-mail: Dongwei0207@163.com

Abstract:

DNA methylation is an important regulator of gene expression during plant development. CUPSHAPED COTYLEDON 1 (CUC1) is a key marker gene during in vitro organogenesis in Arabidopsis thaliana. In this study, the dynamic relation between CUC1 expression and DNA methylation was analyzed by quantitative real-time PCR (qRT-PCR) and bisulfite sequencing PCR (BSP), respectively, during in vitro organogenesis. The expression of CUC1 was negatively correlated with its DNA methylation level in the promoter and 3′ region. This result provides references for further understanding of the possible regulation mechanism of CUC1 in A. thaliana in vitro.

Key words: Arabidopsis thaliana; DNA methylation; CUC1; in vitro organogenesis