《植物生理学报》 2016, 52(6): 810-816

VIGS载体在蔬菜作物中的应用研究进展

季娜娜, 闵德栋, 邵淑君, 李富军, 张新华^*

山东理工大学农业工程与食品科学学院, 山东淄博255049

通信作者：张新华；E-mail: zxh@sdut.edu.cn

摘　要：

病毒诱导的基因沉默(virus-induced gene silencing, VIGS)是一种RNA介导的植物抗病毒机制, 近年来在蔬菜作物基因功能的研究中, VIGS作为一种快速、高效、高通量的反向遗传学新技术发挥了重要作用。由于VIGS技术需要借助病毒载体来实现, 所以选择适宜的病毒载体是在性状各异的蔬菜中成功构建VIGS体系的关键。本文介绍了在蔬菜作物中应用的VIGS载体及其在基因功能研究中的应用实例, 并分析了病毒载体在诱导蔬菜作物基因沉默时存在的一些问题及解决方法。

关键词：VIGS; 病毒载体; 蔬菜; 研究进展

收稿：2016-04-05   修定：2016-05-16

资助：国家自然科学基金(31201432)。

Progress of research on application of VIGS vectors in vegetables

JI Na-Na, MIN De-Dong, SHAO Shu-Jun, LI Fu-Jun, ZHANG Xin-Hua^*

School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong 255049, China

Corresponding author: ZHANG Xin-Hua; E-mail: zxh@sdut.edu.cn

Abstract:

Virus-induced gene silencing (VIGS) is an RNA-mediated antiviral defense mechanism of plant. In recent years, VIGS technology as a fast, effective, high-throughout reverse genetics technology has played an important role in the function study of vegetable gene. VIGS technology is based on the virus vector, so it is the key to selecting a suitable vector for successful implementation of VIGS in a variety of vegetable crops. This paper reviewed the VIGS vectors and its application examples in the gene function research in vegetable. Meanwhile, the problems and its solutions of the application of VIGS in vegetable were analyzed.

Key words: virus-induced gene silencing; virus vectors; vegetable; research progress