甘薯化学离体诱变及突变体的性状表现

张丹丹, 王维华, 李冠, 隋炯明, 乔利仙, 王晶珊, 姜德锋*
青岛农业大学生命科学学院, 山东省高校植物生物技术重点实验室, 山东青岛266109

通信作者:姜德锋;E-mail: dfjiang0535@163.com

摘 要:

利用甘薯品种‘烟薯25’的茎尖为外植体, 平阳霉素作为诱变剂添加于培养基中进行离体诱变培养。4周后, 将形成的愈伤组织转移到不添加平阳霉素的诱导培养基上继续培养, 直到形成不定芽。当不定芽长到2 cm以上后, 从基部切下扦插于MS基本培养基上, 促使其长成完整植株。将再生植株经驯化后移栽田间, 收获期观察发现有3株明显发生变异, 其中一株(P-1)薯块表面光滑无暴筋, 另一株(P-2)暴筋很轻, 而诱变亲本暴筋严重。还有一株(P-3)茎蔓、薯块皮色、肉色均突变为紫色, 但仍保留亲本暴筋严重的遗传性状。第2年进一步鉴定, P-1突变体后代植株性状稳定一致无分离, 所有植株结的薯块表面均无暴筋。P-2突变体后代发生分离。这2个突变体后代平均单株鲜薯重均明显高于诱变亲本。

关键词:甘薯; 平阳霉素; 诱变; 突变体

收稿:2015-12-28   修定:2016-01-17

资助:山东省薯类产业创新团队遗传育种岗位(SDAIT-10-011-0)。

Chemistry mutagenesis in vitro and characteristics of mutants in sweet potato

ZHANG Dan-Dan, WANG Wei-Hua, LI Guan, SUI Jiong-Ming, QIAO Li-Xian, WANG Jing-Shan, JIANG De-Feng*
College of Life Sciences, Qingdao Agricultural University; Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao, Shandong 266109, China

Corresponding author: JIANG De-Feng; E-mail: dfjiang0535@163.com

Abstract:

The stem tips of sweet potato cultivar ‘Yanshu 25’ were cultured on the mutagenic medium supplemented with pingyangmycin (PYM) for 4 weeks. The formed calli were transferred to the induction medium without PYM until the shoots were formed. The shoots above 2 cm were further transferred to the MS basal medium for whole plant formation. The formed plants were transplanted in the field after acclimation in the plastic house. Three mutants were obtained after harvest: one mutant (P-1) produced storage roots having smooth skin and with no reinforced stripes on the surface; another (P-2) showed minor reinforced stripes on the storage root skin, while the storage roots of mutagenic parent ‘Yanshu 25’ have major reinforced stripes on the surface. The third one (P-3) produced storage roots with purple skin, flesh, vines, and still having major reinforced stripes on the surface. In the second generation, the storage roots of P-1 offspring have not reinforced stripes, and no separation was observed. While segregation was observed among the P-2 offsprings, some offsprings produced storage roots with minor reinforced stripes, and others produced storage roots with major reinforced stripes on the surface. The average fresh weights per plant of P-1 and P-2 offsprings were significantly higher than that of mutagenic parent respectively.

Key words: Ipomoea batatas; pingyangmycin; mutagenesis; mutant

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