《植物生理学报》 2015, 51(2): 253-258
通信作者：眭顺照；E-mail: firstname.lastname@example.org, email@example.com；Tel: 023-68250086, 023-68251250
摘 要：为探索乙烯是否参与蜡梅花朵开放衰老进程的调控, 利用气相色谱法测定分析不同发育阶段花朵的乙烯释放情况, 同时分析乙烯、1-甲基环丙烯(1-MCP)处理对切花开放衰老进程和乙烯受体基因表达的影响。结果表明: 蜡梅花朵开放衰老过程中有微量乙烯的产生, 在盛开期出现峰值; 外源乙烯显著加快了花朵开放衰老进程, 缩短切花瓶插寿命1.9 d, 而1-MCP处理则延长瓶插寿命2.4 d; 存在受乙烯和1-MCP影响其在蜡梅花朵中表达的乙烯受体基因成员CpETR-1、CpETR-2、CpETR-3, 且3个基因的转录水平变化与开放衰老进程关联较为紧密。说明蜡梅乙烯释放量虽然很低, 但乙烯参与了蜡梅花朵开放和衰老的调控, 影响其进程和相关乙烯受体基因的表达。
关键词：蜡梅; 切花; 乙烯; 1-MCP; 乙烯受体基因
Corresponding author: SUI Shun-Zhao; E-mail: firstname.lastname@example.org, email@example.com; Tel: 023-68250086, 023-68251250
Abstract:To investigate whether ethylene involved in the regulation of flower opening and senescence in wintersweet, ethylene production of flowers at different stages was measured with gas chromatography. Meanwhile, cut flowers were treated with ethylene and 1-methylcyclopropene (1-MCP), an inhibitor of ethylene receptor, respectively. Then the process of flower opening and senescence was observed and the expression of ethylene receptor genes was analyzed. The results showed that endogenous ethylene was produced slightly, with a maximum amount at full bloom stage of flowers. Exogenous ethylene treatment significantly accelerated the process of flower opening and senescence, resulted in a shortened vase life by 1.9 days compared to the control. In contrast, treatment with 1-MCP extended the vase life by 2.4 days compared to control. Three ethylene receptor genes, CpETR-1, CpETR-2 and CpETR-3, were detected in wintersweet flowers. Their expressions were enhanced by exogenous ethylene and inhibited by 1-MCP treatment, and the changes in the expression of transcriptional level were closely related to the process of flower opening and senescence. These results above indicated that although ethylene production in wintersweet flowers was slight, ethylene also involved in regulation of flower opening and senescence, and influenced the expression of ethylene receptor genes.
Key words: wintersweet (Chimonanthus praecox); cut flower; ethylene; 1-methylcyclopropene (1-MCP); ethylene receptor gene
此摘要已有 2147 人浏览