秀丽野海棠叶片不定芽高频再生体系的建立

洪震1,2, 朱乐杰3, 傅晓强3, 范文峰4, 夏国华3,*
1丽水市林业科学研究院, 浙江丽水323000; 2丽水职业技术学院, 浙江丽水323000; 3浙江农林大学林业与生物技术学院, 浙江临安311300; 4浙江森禾种业股份有限公司, 杭州310007

通信作者:夏国华;E-mail: zjfc_ghxia@126.com;Tel: 0571-63732761

摘 要:

以秀丽野海棠叶片为外植体诱导愈伤组织并分化出不定芽, 得到再生植株, 建立了组织培养和快速繁殖体系。结果表明: 培养基MS+6-BA 1.0 mg•L-1+2,4-D 0.3 mg•L-1适用于叶片外植体愈伤组织诱导和分化, 诱导率为100%, 分化率为85.83%; MS+6-BA 1.0 mg•L-1+IBA 0.3 mg•L-1适用于继代增殖培养, 平均增殖系数为7.38, 且不定芽较粗壮; MS+NAA 0.5 mg•L-1+活性炭1 g•L-1适用于生根培养, 生根率达到100%; 将生长良好的无菌植株进行移栽驯化, 存活率达到90%。

关键词:秀丽野海棠; 叶片; 离体培养; 植株再生

收稿:2014-11-17   修定:2015-01-30

资助:浙江省科技计划项目(2013C32102)、浙江省观赏花卉工程技术研究中心(2012E0036)和丽水市科技计划项目(20120310)。

Establishment of High Frequency Shoot Regeneration System from Leaf of Bredia amoena

HONG Zhen1,2, ZHU Le-Jie3, FU Xiao-Qiang3, FAN Wen-Feng4, XIA Guo-Hua3,*
1Lishui Academy of Forestry, Lishui, Zhejiang 323000, China; 2Lishui Vocational & Technical College, Lishui, Zhejiang 323000, China; 3School of Forestry and Biotechnology, Zhejiang A & F University, Lin’an, Zhejiang 311300, China; 4Zhejiang Senhe Seed Industry Co. Ltd., Hangzhou 310007, China

Corresponding author: XIA Guo-Hua; E-mail: zjfc_ghxia@126.com; Tel: 0571-63732761

Abstract:

In this paper, we studied in vitro culture of Bredia amoena by using leaf explants, including callus induction, adventitious shoot differentiation and plantlet regeneration. The results showed that the best medium for callus induction and adventitious shoot differentiation was MS+6-BA 1.0 mg•L-1+2,4-D 0.3 mg•L-1, with an induction rate of 100% and a differentiation rate of 85.83%. The best multiplication medium was MS+6-BA 1.0 mg•L-1+IBA 0.3 mg•L-1, the multiplication coefficient reached 7.38, and the shoots induced were strong. The optimum rooting medium was MS+NAA 0.5 mg•L-1+activated carbon (AC) 1 g•L-1, and the rooting rate reached 100%. The strong rooting plantlets were transplanted into greenhouse and the survival rate was 90%.

Key words: Bredia amoena; leaf blade; in vitro culture; plantlet regeneration

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