《植物生理学报》 2015, 51(2): 227-232
通信作者：万雪琴；E-mail: firstname.lastname@example.org；Tel: 0835-2882335
摘 要：用添加了不同浓度的AlCl3的培养基培养柳杉组培苗, 研究铝胁迫对柳杉生长及生理特性的影响。结果表明, 与对照相比, 0.5~1.0 mmol•L-1 AlCl3处理能显著提高柳杉组培苗的根长、株高及生物量, 叶中的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)等抗氧化酶活性以及可溶性蛋白含量升高; 当铝浓度处理为1.5 mmol•L-1时, 柳杉的生长受到一定程度抑制, 但叶片内抗氧化酶活性以及可溶蛋白含量等指标仍高于对照, 说明此浓度对柳杉有轻微的胁迫但柳杉能通过自身抗逆境能力缓解伤害; 而在铝浓度达到2.0 mmol•L-1 时, 柳杉生长受到严重抑制, 叶片内丙二醛(MDA)含量显著上升。由此推测, 低浓度铝(<1.0 mmol•L-1)对该柳杉组培苗生长有一定的促进作用, 1.5 mmol•L-1 AlCl3浓度会影响该柳杉组培苗的正常生长, 其耐铝阈值应在1.5~2.0 mmol•L-1之间。
关键词：铝胁迫; 柳杉; 生长特征; 生理特性
Corresponding author: WAN Xue-Qin; E-mail: email@example.com; Tel: 0835-2882335
Abstract:The tissue culture seedlings of Cryptomeria fortunei were treated with different AlCl3 concentrations, in order to investigate effects of aluminum stress on growth and some physiological characteristics of cedar plantlets. The results showed that when compared with the contrast, the treatment of 0.5–1.0 mmol•L-1 AlCl3 could increase the root length, seedling height and biomass effectively, as well as the contents of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and soluble protein in leaves; with 1.5 mmol•L-1 AlCl3, the growth of treated plants were inhibited, but the activities of antioxidases and the content of soluble protein were still higher than contrast, this illustrated that the seedlings were slightly stressed and relieve the damage through their anti-stress capability; the growth of seedlings were inhibited severely and the content of MDA were increased significantly under the treatment of 2.0 mmol•L-1 AlCl3. So we included that lower concentration of aluminum (<1.0 mmol•L-1) could promote the growth of Cryptomeria fortunei tisssue culture seedlings and with the concentration of 1.5 mmol•L-1, the seedlings’ normal growth would be inhibited, the concentration of AlCl3 between 1.5 and 2.0 mmol•L-1 was the threshold for damage.
Key words: aluminum stress; Cryptomeria fortunei; growth traits; physiological features
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