具有ACC脱氨酶活性的海滨锦葵(Kosteletzkya pentacarpos)内生细菌对小麦耐盐性的影响

韩坤*, 田曾元*, 刘珂, 张佳夜, 常银银, 郭予琦**
郑州大学生命科学学院, 郑州450001

通信作者: 郭予琦;E-mail: guoyuqi@zzu.edu.cn;Tel: 0371-67767675

摘 要:

从盐生植物海滨锦葵块根中分离内生细菌43株, 经形态学特征和16S rDNA序列相结合的方法鉴定, 分属10个种属, 其中芽孢杆菌属是优势属, 其次是假单胞菌属和农杆菌属, 蜡样芽孢杆菌(Bacillus cereus)和地衣芽孢杆菌(Bacillus licheniformis)是优势种。对海滨锦葵内生细菌ACC脱氨酶活性的测定显示, 其中5种菌明显具有ACC脱氨酶活性。用筛选到的5种细菌接种盐胁迫下小麦根系并测定其对于小麦耐盐性的影响。结果表明, 蜡样芽孢杆菌、巨大芽孢杆菌(Bacillus megaterium)、短小芽孢杆菌(Bacillus pumilus)、地衣芽孢杆菌四种芽孢杆菌均能显著提高盐胁迫下小麦幼苗的干物质重和叶绿素含量, 并能显著提高保护酶(SOD、POD、CAT)活性, 对盐胁迫的毒害有一定的缓解作用。绿针假单胞菌(Pseudomonas chlororaphis)对小麦幼苗株高、根长、鲜重、干重、叶绿素含量和保护酶活性的提高也具有一定的作用。上述分析表明从海滨锦葵块根中分离出的5株具有ACC脱氨酶活性的内生细菌均能提高小麦幼苗的耐盐性。

关键词:内生菌; 海滨锦葵; 小麦; 盐胁迫; ACC脱氨酶

收稿:2014-11-14   修定:2014-12-29

资助:国家“948”计划项目(2011-Z30)。 * 并列第一作者。 ** 通讯作者。

Effect of Endophytic Bacteria with ACC Deaminase Activity in Kosteletzkya pentacarpos on Wheat Salt Tolerance

HAN Kun*, TIAN Zeng-Yuan*, LIU Ke, ZHANG Jia-Ye, CHANG Yin-Yin, GUO Yu-Qi**
School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China

Corresponding author: HAN Kun; E-mail: guoyuqi@zzu.edu.cn; Tel: 0371-67767675

Abstract:

43 endophytic bacteria strains, isolated from roots of the halophyte Kosteletzkya pentacarpos, belong to ten genus by 16S rDNA sequencing and the identification of morphological features. Among them, Bacillus is the advantage genus, and then is Pseudomonas and Agrobacterium. Bacillus cereus and Bacillus licheniformis are the advantage species. ACC deaminase activity of endophytic bacteria in Kosteletzkya pentacarpos has been determined. Results revealed that five strains could produce obvious ACC deaminase activity. These five kinds of bacteria were used to inoculate wheat roots under salt stress to determine their effects on the salt resistance of wheat. Data showed that four strains, including Bacillus cereus, Bacillus megaterium, Bacillus pumilus and Bacillus licheniformis, could not only promote the wheat fresh weight, dry weigh and chlorophyll content under salt stress, but also significantly raise enzyme activities of SOD, POD and CAT, showing elite alleviating capacity to the salt toxicity. Additionally, Pseudomonas chlororaphis, to a certain extent, also showed the promotive effects on wheat plant height, root length, fresh weight, dry weight and chlorophyll content. These suggested that all the five endophytic bacteria isolated from Kosteletzkya pentacarpos with ACC deaminase can enhance the salt resistance of wheat seedings.

Key words: endophyte; Kosteletzkya pentacarpos; wheat; salt stress; ACC deaminase

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