同源四倍体拟南芥的耐锌性及其机理

任冲, 徐国云, 李明娟, 崔延春, 王曼玲, 夏新界*
中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙410125

通信作者:夏新界;E-mail: jxxia@isa.ac.cn;Tel: 0731-4619769

摘 要:

锌(Zn)是植物生长发育所必需的微量元素, 但其过量积累也会对植物产生毒害作用。通过对同源四倍体拟南芥进行高浓度锌处理, 发现四倍体拟南芥对锌具有高耐受性和低积累性的特点。在高浓度锌胁迫条件下, 同源四倍体的根长和生物量较野生型对照均有明显增加, 并且谷胱甘肽(GSH)的含量也明显升高。定量实时PCR (qRT-PCR)分析表明, 一些锌胁迫响应基因的表达水平发生了显著改变。以上结果表明, 锌胁迫响应基因表达水平的改变以及GSH含量的升高可能在四倍体拟南芥的耐锌过程中发挥重要作用。

关键词:同源四倍体拟南芥; 耐锌性; 基因表达; 谷胱甘肽

收稿:2014-12-03   修定:2014-12-24

资助:国家自然科学基金(31401314和31171536)。

Zinc Tolerance and its Mechanism in Autotetraploid Arabidopsis thaliana

REN Chong, XU Guo-Yun, LI Ming-Juan, CUI Yan-Chun, WANG Man-Ling, XIA Xin-Jie*
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China

Corresponding author: XIA Xin-Jie; E-mail: jxxia@isa.ac.cn; Tel: 0731-4619769

Abstract:

Zinc (Zn) is an essential micronutrient for plants, but it becomes toxic when accumulated in excess. An autotetraploid Arabidopsis thaliana treated with high concentration of Zn was found to be Zn-tolerant without Zn-hyperaccumulation. The root length and biomass of autotetraploid plants exhibited increases upon excess Zn exposure, compared to wild-type plants, and the contents of total glutathione (GSH) were observed to be increased as well in autotetraploid plants. Quantitative real-time PCR (qRT-PCR) analysis of the expression profiles of autotetraploid plants revealed the alterations of some Zn stress-responsive genes in their expression levels. The obtained results suggested that altered expressions of Zn-responsive genes and increased GSH content may play a major role in the Zn tolerance of autotetraploid Arabidopsis.

Key words: autotetraploid Arabidopsis; zinc tolerance; gene expression; glutathione

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