Cu2+对铜锈微囊藻生长及叶绿素荧光特性的影响

晋利, 李晓亮*
中国环境管理干部学院环境工程系, 河北秦皇岛066102

通信作者:李晓亮;E-mail: xll1979@gmail.com;Tel: 0335-7815875

摘 要:

利用浮游植物荧光仪(Photo-PAM)研究了不同Cu2+浓度处理不同时间(12、24、48、72、96 h)对铜锈微囊藻(Microcystis aeruginosa)生长及叶绿素荧光特性的影响。结果表明: 在一定时间(96 h)内, 低浓度Cu2+ (1 μmol•L-1)处理的铜锈微囊藻的藻细胞密度以及PSII最大光合效率(Fv/Fm)、PSII实际光合效率(ΦPSII)、光能利用效率(α)与对照相比变化不显著, 最大电子传递速率(Pm)显著下降; 高浓度Cu2+ (2、4、6、8 μmol•L-1)处理下, 藻细胞密度及Fv/FmΦPSII、α、Pm随着Cu2+浓度的加大和胁迫时间的延长均显著降低, 浓度为6、8 μmol•L-1 Cu2+处理48 h, 铜锈微囊藻Fv/FmΦPSII、α、Pm的测得值为0。可见, 高浓度Cu2+ (≥2 μmol•L-1)处理极大地影响铜锈微囊藻的生长及叶绿素荧光特性, 胁迫作用显著。

关键词:Cu2+; 铜锈微囊藻; 生长; 叶绿素荧光

收稿:2014-11-24   修定:2015-01-29

资助:国家自然科学基金(41201244)和中国环境管理干部学院博士基金(B201409)。

Effects of Cu2+ on the Growth and Chlorophyll Fluorescence of Microcystis aeruginosa

JIN Li, LI Xiao-Liang*
Environmental Engineering Department, Environmental Management College of China, Qinhuangdao, Hebei 066102, China

Corresponding author: LI Xiao-Liang; E-mail: xll1979@gmail.com; Tel: 0335-7815875

Abstract:

Algae, live in body of water, are inevitably stressed by copper pollution. The growth and chlorophyll fluorescence traits of Microcystis aeruginosa exposed to the culture medium with different copper concentrations for different times (12, 24, 48, 72, 96 h) were studied. The results showed that the maximal photochemical efficiency of PSII (Fv/Fm), the actual photochemical efficiency of PSII in the light (ΦPSII) and the light use efficiency (α) were no significant differences under low Cu2+ concentration (1 μmol•L-1), but the maximal relative electron transport rate (Pm) was decreased significantly within 96 hours. However, when Cu2+ concentrations were high (2, 4, 6 and 8 μmol•L-1), the differences of the parameters were significant. And the changes of the decrease tendency were evident with the increase of Cu2+ concentrations and the prolongation of time. The measured values of Fv/Fm, ΦPSII, α and Pm were zero when Cu2+ concentrations were 6 and 8 μmol•L-1 after 48 h. Therefore, the high content of Cu2+ (≥2 μmol•L-1) inhibited the growth and chlorophyll fluorescence of M. aeruginosa severely.

Key words: Cu2+; Microcystis aeruginosa; growth; chlorophyll fluorescence

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