盐胁迫下黄芩种子萌发及幼苗对外源抗坏血酸的生理响应

江绪文1, 李贺勤1,*, 王建华2
1青岛农业大学农学与植物保护学院, 山东省旱作农业技术重点实验室, 山东青岛266109; 2中国农业大学农学与生物技术学院, 种子科学研究中心, 北京100193

通信作者:李贺勤;E-mail: hqliaau@163.com;Tel: 0532-86080447

摘 要:

以黄芩(Scutellaria baicalensis)种子为材料, 用氯化钠(NaCl)模拟盐胁迫条件, 采用纸上发芽, 研究外源抗坏血酸(AsA)对盐胁迫下黄芩种子萌发及幼苗的影响。结果表明, 在80 mmol•L-1 NaCl胁迫下, 黄芩种子萌发及幼苗生理指标受到显著抑制; 0.50 mmol•L-1 AsA处理可显著提高盐胁迫下黄芩种子的发芽势(GE)、发芽率(GR)、发芽指数(GI)、简化活力指数(SVI)以及根系总黄酮、可溶性糖和脯氨酸(Pro)含量, 同时也可显著提高幼苗根系活力和超氧化物歧化酶(SOD)活性, 显著降低丙二醛(MDA)含量。这说明适宜浓度的抗坏血酸能提高黄芩种子的萌发能力和幼苗对盐胁迫的适应能力, 从而起到缓解盐胁迫对种子萌发及幼苗生长的抑制作用。

关键词:黄芩; 抗坏血酸; 盐胁迫; 种子萌发; 幼苗; 生理响应

收稿:2014-11-10   修定:2015-01-04

资助:公益性行业(农业)科研专项(201303002)和山东省旱地作物水分高效利用创新团队项目(62112N5)。

Physiological Response of Scutellaria baicalensis Seed Germination and Seedling to Exogenous Ascorbic Acid under Salt Stress

JIANG Xu-Wen1, LI He-Qin1,*, WANG Jian-Hua2
1Shandong Provincial Key Laboratory of Dryland Technology, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, Shandong 266109, China; 2Seed Science Research Center, College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China

Corresponding author: LI He-Qin; E-mail: hqliaau@163.com; Tel: 0532-86080447

Abstract:

Seed germination and seedling physiological characteristics of Scutellaria baicalensis were studied with exogenous ascorbic acid under NaCl stress by paper germination. The results showed that seed germination and seedling physiological characteristics of S. baicalensis were significantly inhibitied under 80 mmol•L-1 NaCl stress. Compared with NaCl stress, germination energy, germination rate, germination index, simplified vigor index, total flavonoids content, soluble sugar content and proline content, SOD activity in roots and root vigor were significantly improved with 0.50 mmol•L-1 ascorbic acid, as well as MDA content was significantly reduced. The above results indicated that seed germination and seedling growth of S. baicalensis were improved by the optimum concentration of exogenous ascorbic acid to alleviate the inhibitory effect under NaCl stress.

Key words: Scutellaria baicalensis; ascorbic acid; NaCl stress; seed germination; seedling; physiological response

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