超声处理对霍山石斛类原球茎悬浮培养细胞生理代谢的影响

魏明1,*, 杨超英1, 姜绍通2
1安徽工程大学生物与化学工程学院, 安徽芜湖241000; 2合肥工业大学生物与食品工程学院, 合肥230009

通信作者:魏明;E-mail: wmrainbow@yahoo.com.cn;Tel: 0553-2876254

摘 要:

为了了解超声处理对霍山石斛类原球茎产生的生理效应, 研究了超声波功率和超声时间对霍山石斛类原球茎悬浮培 养细胞生长以及多糖和蛋白质合成的影响; 分析了培养基中碳、氮利用、细胞内活性氧水平以及蔗糖转化酶、硝酸还原 酶、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性的变化。结果表明, 适当功率和时间的超声处理 (300 W, 3 min)能显著促进霍山石斛类原球茎的增殖, 最大细胞干重为34.6 g•L-1; 明显促进培养基中碳和氮的利用; 显著提 高胞内可溶性多糖、可溶性蛋白质和H2O2的含量; 细胞内蔗糖转化酶、硝酸还原酶以及SOD、CAT和POD的活性明显升 高。适当的超声波处理能促进霍山石斛类原球茎的生长发育和提高细胞的生理活性。

关键词:霍山石斛; 类原球茎; 超声波; 生理活性

收稿:2011-05-10   修定:2011-05-10

资助:教育部科学技术研究重点项目(03098)。

Effect of Ultrasonic on the Physiological Metabolism of Cells in Suspension Cultures of Protocorm-Like Bodies of Dendrobium huoshanense

WEI Ming1,*, YANG Chao-Ying1, JIANG Shao-Tong2
1College of Biology and Chemistry, Anhui Polytechnic University, Wuhu, Anhui 241000, China; 2School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China

Corresponding author: WEI Ming; E-mail: wmrainbow@yahoo.com.cn; Tel: 0553-2876254

Abstract:

In order to find out the physiological effects of ultrasonic on the cells in suspension cultures of protocorm-like bodies (PLBs) of Dendrobium huoshanense. The effects of ultrasonic power and time on PLB proliferation and synthesis of polysaccharides and proteins were investigated. The nutrient utilization in the medium, reactive oxygen species (ROS), the activities of invertase, nitrate reductase, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in the cells of PLB were analyzed. The results indicated that the optimum ultrasonic power and time (300 W, 3 min) could significantly enhance the cell growth and were beneficial to the utilization of carbon and nitrogen. The cell dry weight reached 34.6 g (DW)·L-1. The contents of soluble polysaccharides, proteins and H2O2 in cells were increased greatly. Invertase, nitrate reductase, SOD, CAT and POD activities were found to increase significantly in the cultured cells treated with ultrasonic. The suitable ultrasonic treatment was beneficial to the cell growth and the physiological activity of PLBs in suspension cultures of PLBs of D. huoshanense.

Key words: Dendrobium huoshanense; protocorm-like bodies; ultrasonic; physiological activity

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