大麦DH矮杆突变体的表型分析及其对外源赤霉酸的响应

高润红1,2,*, 郭桂梅1,2,*, 徐红卫1,2, 陈志伟1,2, 刘成洪1,2, 黄亦辰1,2,3, 何婷1,2, 李颖波1,2, 陆瑞菊1,2, 黄剑华1,2,**
1上海市农业科学院生物技术研究所, 上海201106; 2上海市农业遗传育种重点实验室, 上海201106; 3上海海洋大学水产与生命学院, 上海201306

通信作者:高润红;E-mail: sw1@saas.sh.cn;Tel: 021-62201032

摘 要:

利用物理诱变结合小孢子培养创造新的变异可以为作物遗传改良提供新的遗传资源。本研究利用60Co γ-射线辐照优良大麦品种‘花30’和‘花11’干种子, 取其植株上花药, 游离小孢子培养获得3份纯合的矮杆突变体。突变体的株高为亲本的52.4%~61.1%, 株高降低效应显著。苗期以及拔节期赤霉酸处理表明突变体及野生型均为赤霉酸敏感型, 而且不同突变体对赤霉酸反应的敏感性不同。

关键词:大麦; 突变体; 矮杆; 赤霉酸

收稿:2014-10-15   修定:2014-11-21

资助:上海市科委基础研究重点项目(12JC1407800)、上海市种业发展项目[沪农科种字(2012)第7号]、国家大麦青稞产业技术体系(CARS-05)和上海市农业科学院学科建设专项(LY11)。 * 并列第一作者。

Phenotypic Analysis and Gibberellic (GA3) Response of Barley Double Haploid Dwarf Mutants

GAO Run-Hong1,2,*, GUO Gui-Mei1,2,*, XU Hong-Wei1,2, CHEN Zhi-Wei1,2, LIU Cheng-Hong1,2, HUANG Yi-Chen1,2,3, HE Ting1,2, LI Ying-Bo1,2, LU Rui-Ju1,2, HUANG Jian-Hua1,2,**
1Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106, China; 3College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

Corresponding author: GAO Run-Hong; E-mail: sw1@saas.sh.cn; Tel: 021-62201032

Abstract:

Physical mutagenesis combined with microspore culture can create the germplasm with new variation for crop improvement. In this study, three homogeneous dwarf mutants of two barley cultivars ‘Hua30’ and ‘Hua11’ were obtained through isolated microspore culture on the plants derived from 60Co γ-rays irradiated seeds. The dwarf mutants showed a significant reduction on plant height, which was 52.4%–61.1% of the wild types. Both the mutants and their wild types were sensitive to exotic GA3 treatment at seedling and elongation stages, however, the mutants revealed different sensibility to GA3.

Key words: barley; mutant; dwarf; GA3

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