《植物生理学报》 2011, 47(6): 575-580

粗毛淫羊藿的类黄酮异戊烯转移酶基因的克隆与表达分析

陈倩倩^1,3, 高娟¹, 赵沛基¹, 卢山², 曾英^1,*

1中国科学院昆明植物研究所, 植物化学与西部植物资源持续利用国家重点实验室, 昆明 650204; 2南京大学生命科学学院,

通信作者：曾英；E-mail: biochem@mail.kib.ac.cn；Tel: 0871-5223210

摘　要：

药用植物淫羊藿富含异戊烯类黄酮, LC-MS定量分析表明, 3~4月份粗毛淫羊藿叶片处于变色期时淫羊藿苷含量最 高。通过简并引物和RACE-PCR从粗毛淫羊藿叶片获得异戊烯转移酶同源基因(命名为EaPT1)。序列分析表明, EaPT1与 维生素E合成相关的尿黑酸异戊烯转移酶位于同一进化枝, 靠近类黄酮异戊烯转移酶。RT-PCR结果显示, EaPT1在叶片和 幼茎中表达显著。

关键词：淫羊藿苷; 异戊烯转移酶; 异戊烯类黄酮; 粗毛淫羊藿

收稿：2011-01-06   修定：2011-01-06

资助：“973”计划(2007CB108802)和植物化学与西部植物资源 持续利用国家重点实验室自主课题(P2008-ZZ16和P2009- ZZ02)。

Cloning and Expression Analysis of Flavonoid Prenyltransferase-Like Genes in Epimedium acuminatum Franch

CHEN Qian-Qian^1,3, GAO Juan¹, ZHAO Pei-Ji¹, LU Shan ², ZENG Ying^1,*

¹State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; ²School of Life Science, Nanjing University, Nanjing 210093, China; ³Graduate University of the Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: ZENG Ying; E-mail: biochem@mail.kib.ac.cn; Tel: 0871-5223210

Abstract:

The traditional Chinese medicinal plant, Epimedium acuminatum, is remarkably rich in pharmacologically active prenylflavonoids. LC-MS analysis indicated that the leaves of Epimedium acuminatum had the highest icariin content in the color phase (from March to April). A prenyltransferase-like gene (named EaPT1) was cloned from those leaves with degenerate primers and subsequent rapid amplification of cDNA ends (RACE). The deduced protein sequence of EaPT1 grouped together with homogentisate phytyltransferases relevant to vitamin E biosynthesis and closely related to the flavonoid prenyltransferases. The EaPT1 transcripts significantly accumulated in leaves and young stems by RT-PCR.

Key words: icariin; prenyltransferase; prenylflavonoid; Epimedium acuminatum