利用SRAP标记分析中国主栽孔雀草品种的遗传多样性

李春楠1,*, 傅巧娟1, 陈一1, 赵福康1, 孙瑶1, 崔海瑞2,*
1杭州市农业科学研究院园艺研究所, 杭州310024; 2浙江大学原子核农业科学研究所, 农业部核农学重点开放实验室, 杭州310029

通信作者:李春楠;E-mail: lcn0814@126.com,hrcui@zju.edu.cn;Tel: 0571-87313243, 0571-86971405

摘 要:

为了解中国主栽孔雀草品种的遗传背景, 采用相关序列扩增多态性(SRAP)分子标记分析了28份孔雀草材料的遗传多样性。14对SRAP引物组合共获得271个位点, 其中多态位点151个, 占55.72%。每对引物可扩增出14~24条DNA片段, 平均19.4条。引物的多态信息含量PIC值在0.693~0.967之间, 平均为0.909; 每个材料得到的多态性条带比例介于38.78%与51.42%之间, 平均46.38%, 说明SRAP分子标记可有效鉴别孔雀草种质在分子水平上的遗传变异。品种间的遗传距离值在0.047~0.198之间, 平均为0.126; Shannon多样性指数变化于0.178~0.217之间, 平均0.201, 表明参试的孔雀草材料总体的遗传多样性水平较低。UPGMA聚类后, 在遗传距离阈值为0.146处, 可将28份材料分为4大类群, 与花色表现基本相符, 花色可考虑作为孔雀草基于表型分类的主要因子。本研究结果对孔雀草品种鉴定、杂交育种中亲本选配和分子标记辅助选择具有重要意义。

关键词:孔雀草; 遗传多样性; SRAP; 聚类

收稿:2014-05-28   修定:2014-06-30

资助:杭州市科技发展计划项目(20120332H03)

Analysis of Genetic Diversity of the Major Tagetes patula Varieties in China Using SRAP Markers

LI Chun-Nan1,*, FU Qiao-Juan1, CHEN Yi1, ZHAO Fu-Kang1, SUN Yao1, CUI Hai-Rui2,*
1Institute of Horticulture, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China; 2Key Laboratory of Nuclear Agricultural Sciences, Ministry of Agriculture, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, China

Corresponding author: LI Chun-Nan; E-mail: lcn0814@126.com,hrcui@zju.edu.cn; Tel: 0571-87313243, 0571-86971405

Abstract:

In order to understand the genetic variation of Tagetes patula varieties in China, the genetic diversity of 28 major cultivars was assessed using SRAP (sequence-related amplified polymorphism) markers. A total of 271 scorable fragments were identified with 14 primer combinations, of which 151 (55.72%) were polymorphic. Each primer pairs amplified 14 to 24 DNA bands with an average of 19.4 bands. The polymorphism information content (PIC) for each primer pair varied from 0.693 to 0.967 with a mean of 0.909 and the percentage of polymorphic fragments for each variety ranged from 38.78% to 51.42% with an average of 46.38%. These parameters suggest that the SRAP marker could effectively identify the genetic variation among T. patula varieties. The genetic distance among varieties ranged from 0.047 to 0.198 with an average of 0.126 and Shannon diversity index varied from 0.178 to 0.217 with an average of 0.201, indicating the low degree of genetic diversity among these cultivars tested. The 28 cultivars could be divided into 4 groups when the genetic distance was 0.146 based on UPGMA cluster analysis, basically corresponding to the flower color, and it could be considered as a predominant factor for the cluster of T. patula in terms of phenotypic traits. These data may provide a base for cultivar identification, parent selection and marker assisted selection (MAS) in crossbreeding of T. patula.

Key words: Tagetes patula; genetic diversity; SRAP; cluster

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